海滨锦葵胚轴愈伤组织诱导及植株再生

植物学报 ›› 2007, Vol. 24 ›› Issue (02): 194-199.

海滨锦葵胚轴愈伤组织诱导及植株再生

郑熙王学英单莹金华阮成江

1 沈阳农业大学生物科学技术学院, 沈阳 110161; 2 大连民族学院生命科学学院, 大连 116600

收稿日期:2006-07-18 修回日期:2006-11-14 出版日期:2007-03-01 发布日期:2007-03-01
通讯作者: 阮成江

Callus Induction and Plant Regeneration from Embryo Exes of Kosteletzkya virginica

Xi Zheng, Xueying Wang, Ying Shan, Hua Jin, Chengjiang Ruan

1Biological Science and Technology College, Shenyang Agriculture University, Shenyang 110161, China;2College of Life Sciences, Dalian Nationalities University, Dalian 116600, China

Received:2006-07-18 Revised:2006-11-14 Online:2007-03-01 Published:2007-03-01
Contact: Chengjiang Ruan

摘要/Abstract

摘要： 以海滨锦葵(Kosteletzkya virginica)胚轴为外植体, 在9种不同激素配比的培养基上进行愈伤组织诱导、继代培养、不定芽分化及生根培养, 确定了植株再生的最适培养条件: (1)愈伤组织诱导最适培养基为MS + IAA 1.0 mg.L-1 + KT 0.3 mg.L-1 + sucrose 30 g.L-1 + agar 8 g.L-1, 愈伤组织诱导率为93.94%; (2)不定芽诱导最适培养基为MS + IAA 0.1 mg.L-1 + ZT 0.5 mg.L-1 + sucrose 30 g.L-1 + agar 8 g.L-1, 不定芽诱导率为65.83%; (3)生根最适培养基为MS + sucrose 30 g.L-1 +agar 8 g.L-1, 生根率为96.67%。炼苗移栽后, 成活率可达85%。

Abstract: The embryo exes of Kosteletzkya virginica were used as explants for induction of callus, buds and roots on nine culture media with different hormone combinations. The best results were achieved on the following media: (1) 93.94% callus induction on MS medium supplemented with 1.0 mg.L-1 IAA, 0.3 mg.L-1 KT, 30 g.L-1 sucrose and 8 g.L-1 agar; (2) 65.83% shoot induction on MS medium supplemented with 0.1 mg.L-1 IAA, 0.5 mg.L-1 ZT, 30 g.L-1 sucrose and 8 g.L-1 agar; and (3) 96.67% rooting on MS medium containing 30 g.L-1 sucrose and 8 g.L-1 agar. The survival rate of the plantlets was 85% after being transplanted into soil.

郑熙王学英单莹金华阮成江. 海滨锦葵胚轴愈伤组织诱导及植株再生. 植物学报, 2007, 24(02): 194-199.

Xi Zheng;Xueying Wang;Ying Shan;Hua Jin;Chengjiang Ruan. Callus Induction and Plant Regeneration from Embryo Exes of Kosteletzkya virginica. Chinese Bulletin of Botany, 2007, 24(02): 194-199.

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