Abstract: To analyze the heredity of tuberculate trait in Cucumis sativus, we constructed 2 populations. Female parents were 2 varieties without tuberculate, Z1 and Z3, derived from Dutch species, and planted in a greenhouse. The male parent was Dongnong 129, a variety with tuberculate. The fruit of the F1 family from the 2 crosses had tuberculate, which indicated that the tuberculate trait was dominant. The tuberculate trait and no tuberculate trait segregated in the F2 family. The segregation ratios were 2.92 : 1 and 2.95 : 1 for the 2 families, respectively, which revealed that the gene Tu played a role in independent inheritance. A total of 86 pairs of simple sequence repeat primers were screened in 2 near-isogenic pools created by mixing DNA of 10 individuals randomly chosen from the F2 family according to tuberculate or no tuberculate trait. Five separate amplification products obtained by use of 5 pairs of the primers and 4 products — CSWGATT01B, CSWGATT01C, CSCT335, and CSWGATT01A — were mapped to the same linkage group by use of MAPMAKER/EXP, version 3.0b. Tu in the linkage group located between CSWGATT01C and CSCT335 at 20.0 cM and 14.1 cM from the markers, respectively.