植物学报 ›› 2006, Vol. 23 ›› Issue (4): 389-394.
• 技术与方法 • 上一篇 下一篇
景新明 尹广鹍
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Xinming Jing†, Guangkun Yin
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摘要： 利用不连续Percoll梯度分离得到高纯度的大豆(Glycine max)种子线粒体。线粒体制剂中没有检测到胞液、过氧化物体和叶绿素的污染。线粒体的完整性达到97%以上, 在0~4 ℃下至少稳定2小时。
Abstract: A procedure for isolating high pure mitochondria from soybean (Glycine max) seeds by discontinuous Percoll density gradient centrifugation was established. The preparation contains more than 97% mitochondria as intact organelles with no detectable cytosol, peroxisomes and chlorophyll contamination. The mitochondria are stable at 0-4℃ for at least 2 hours.
景新明 尹广鹍. 高纯度大豆种子线粒体的分离. 植物学报, 2006, 23(4): 389-394.
Xinming Jing&#;Guangkun Yin. Isolation of High Pure Mitochondria from Soybean Seeds. Chinese Bulletin of Botany, 2006, 23(4): 389-394.
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