拟南芥中一个未知功能蛋白的叶绿体亚细胞定位研究

植物学报 ›› 2006, Vol. 23 ›› Issue (3): 249-254.

拟南芥中一个未知功能蛋白的叶绿体亚细胞定位研究

王鹏程王晨米华玲周根余杨仲南

(1 上海师范大学生命与环境科学学院上海 200234) (2 中国科学院上海植物生理生态研究所上海 200032)

收稿日期:2005-12-20 修回日期:2006-03-07 出版日期:2006-04-15 发布日期:2006-04-15
通讯作者: 杨仲南

Study of Subcellular Localization of the Expressed Protein in Arabidopsis thaliana

Pengcheng Wang, Chen Wang, Hualing Mi, Genyu Zhou, Zhongnan Yang

1(College of Life and Envionment Science, Shanghai Normal University, Shanghai 200234) 2(Shanghai Institute Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032)

Received:2005-12-20 Revised:2006-03-07 Online:2006-04-15 Published:2006-04-15
Contact: Zhongnan Yang

摘要/Abstract

摘要： 生物信息学分析表明, 模式植物拟南芥叶绿体中含有大约4 000多种蛋白质, 目前只分离得到1 000多种, 其他预测的叶绿体蛋白的实验验证对叶绿体功能研究有重要意义。本文对一个预测的叶绿体未知功能蛋白AT5G48790进行了亚细胞定位研究。我们克隆了该基因5'端长178 bp的DNA片段, 与绿色荧光蛋白(GFP)基因构建重组载体pMON530-cTP-GFP。转基因植株通过激光共聚焦显微镜观察, GFP只在叶绿体中特异表达。实验结果表明, AT5G48790的确为叶绿体蛋白。本实验方法也可用于其他预测的蛋白质的实验验证。

Abstract: Bioinformatics analysis has revealed about 4 000 proteins in chloroplasts; however, only about 1 000 proteins have been validated. Thus, we established an experimental system to verify predicted chloroplast proteins. At5g48790 was predicted to be an Arabidopsis chloroplast protein.The 178 bp segment of the 5' sequence of this gene was cloned and fused with GFP to construct a binary vector pMON530-cTP-GFP for genetic transformation. On confocal laser-scanning microscopy, green fluorescent signals were localized in chloroplasts in transgenic Arabidopsis plants. The results suggest that At5g48790 encodes a chloroplast protein. This experiment can also be used to investigate other proteins predicted to be located in chloroplasts in Arabidopsis．

王鹏程王晨米华玲周根余杨仲南. 拟南芥中一个未知功能蛋白的叶绿体亚细胞定位研究. 植物学报, 2006, 23(3): 249-254.

Pengcheng Wang;Chen Wang;Hualing Mi;Genyu Zhou;Zhongnan Yang. Study of Subcellular Localization of the Expressed Protein in Arabidopsis thaliana. Chinese Bulletin of Botany, 2006, 23(3): 249-254.

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