植物学报 ›› 2006, Vol. 23 ›› Issue (1): 52-55.
• 组织培养简讯 •
赵术珍 阮圆 王宝山
Shuzhen Zhao, Yuan Ruan, Baoshan Wang
摘要： 选用盐地碱蓬(Suaeda salsa)幼嫩花序为外植体, 建立了快速而高效的离体培养体系。在附加1.0mg.L-16-BA和0.4 mg.L-1 IAA的MS培养基上培养25天可诱导出不定芽, 诱导频率达到82.1%; 不定芽在此培养基上可快速扩增和长期继代培养。不定芽转至MS培养基中培养2~3周生根形成完整植株。
Abstract: A protocol for high frequency adventitious shoot induction and plantlet regeneration from immature inflorescence explants of Suaeda salsa (a C3 halophyte) was reported. Adventitious shoots were induced from immature inflorescence cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg.L-1 6-benzylaminopurine (6-BA) and 0.4 mg.L-1 indole-3-acetic acid (IAA) for 25 days. Induction frequency of adventitious shoots was about 82.1%. Adventitious shoots were further multiplied vigorously and maintained for a long time on the same medium for adventitious shoot induction. Rooting was achieved on MS medium without any plant growth regulators after adventitious shoot was transplanted for 2-3 weeks.
赵术珍 阮圆 王宝山. 盐地碱蓬幼嫩花序的组织培养及植株再生. 植物学报, 2006, 23(1): 52-55.
Shuzhen Zhao;Yuan Ruan;Baoshan Wang. Tissue Culture and Plant Regeneration from Immature Inflorescence Explants of Suaeda salsa. Chinese Bulletin of Botany, 2006, 23(1): 52-55.