细胞融合与植物细胞质雄性不育性状改良

植物学报 ›› 2005, Vol. 22 ›› Issue (增刊): 99-107.

细胞融合与植物细胞质雄性不育性状改良

付春华余龙江

(华中科技大学生命科学与技术学院武汉 430074)

收稿日期:2004-05-20 修回日期:2004-12-22 出版日期:2005-08-10 发布日期:2005-08-10
通讯作者: 付春华
基金资助:
华中科技大学引进人才启动项目(0101170064)资助

Research Progress in Plant Cytoplasmic Male Sterility by Protoplast Fusion

FU Chun-Hua YU Long-Jiang

(College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074)

Received:2004-05-20 Revised:2004-12-22 Online:2005-08-10 Published:2005-08-10

摘要/Abstract

摘要： 原生质体融合技术不仅可以实现核基因组的重组, 而且可以实现胞质基因组的重组, 为转移由线粒体DNA或叶绿体DNA控制的性状提供了一条捷径。本文综述了获得胞质杂种的方法及其原理, 细胞融合在胞质转移及创造新的细胞质雄性不育(CMS)资源上的研究进展及其应用情况, 以及CMS在有性后代中的遗传规律, 探讨了此育种途径的优势及其在木本果树无籽育种上的应用。

关键词: 原生质体融合, 细胞质雄性不育(CMS), 胞质杂种, 无籽育种

Abstract: Protoplast fusion for combining and exchanging nuclear and cytoplasm genomes in hybrid progeny provides a new strategy to improve traits controlled by chloroplast or mitochondrial DNA. This paper reviews the methods and mechanisms of obtaining cybrids, advances in transforming cytoplasmic male sterility (CMS), the potential use of these CMS somatic hybrids and the genetic transformation of CMS in sex hybrids. In addition, the application of this strategy to breeding seedless woody fruit trees is discussed.

Key words: Protoplast fusion, Cytoplasmic male sterility (CMS), Cybrids, Seedless breeding

付春华余龙江. 细胞融合与植物细胞质雄性不育性状改良. 植物学报, 2005, 22(增刊): 99-107.

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