植物学报 ›› 2005, Vol. 22 ›› Issue (增刊): 64-67.

• 实验简报 • 上一篇    下一篇

野生莲资源的RAPD 分析

郭宏波 柯卫东 李双梅 彭静   

  1. (武汉市蔬菜科学研究所 武汉 430065)
  • 收稿日期:2004-07-05 修回日期:2004-12-29 出版日期:2005-08-10 发布日期:2005-08-10
  • 通讯作者: 柯卫东

Analysis of Wild Lotus with RAPD Markers

GUO Hong-Bo KE Wei-Dong LI Shuang-Mei PENG Jing   

  1. (Wuhan Vegetable Institute of Science, Wuhan 430065)
  • Received:2004-07-05 Revised:2004-12-29 Online:2005-08-10 Published:2005-08-10

摘要: 利用16个随机引物对来自6省的12份野生莲(Nelumbo nucifera)资源材料进行了RAPD分析。扩增形成141条谱带, 其中多态带79条, 占56.03%。显示野生莲在我国具有丰富的遗传多样性。结果还表明: 1)野生莲资源遗传分化十分明显, 虽有一定的地域相似性, 但有些地区的分化也很明显; 2)野生莲资源可分为3类, 与园艺学分类比较相似, 可能暗示了现代莲由不同遗传背景的野生莲演化而来。

关键词: 野生莲(Nelumbo nucifera), RAPD, 聚类分析

Abstract: RAPD analysis of 12 samples of wild lotus (Nelumbo nucifera) from 6 provinces showed that wild lotus have rich biodiversity and significant difference in markers, especially those from Hubei province. All materials could be divided into 3 groups, which is similar to classification by horticultural characteristics. This analysis indicated that the current 3 types of lotus cultivars might be derived from different wild lotus.

Key words: Wild lotus ( Nelumbo nucifera), RAPD, Cluster analysis