差异显示技术研究NaHCO3 胁迫下星星草基因表达

植物学报 ›› 2005, Vol. 22 ›› Issue (03): 307-312.

差异显示技术研究NaHCO3 胁迫下星星草基因表达

王玉成,杨传平,刘桂丰,姜静

(东北林业大学林学院哈尔滨 150040)

收稿日期:2004-01-07 修回日期:2004-07-05 出版日期:2005-06-20 发布日期:2005-06-20
通讯作者: 王玉成 Author for correspondence. E-mail: wangyucheng1029@yahoo.com.cn
基金资助:
国家 973 计划项目(G1999016000)和国家转基因植物研究与产业化专项(JY03A1802)资助

Studies of Gene Expression in Puccinellia tenuiflora under NaHCO3 Stress Using Differential Display Technology

WANG Yu-Cheng YANG Chuan-Ping LIU Gui-Feng JIANG Jing

(College of Forestry, Northeast Forestry University, Harbin 150040)

Received:2004-01-07 Revised:2004-07-05 Online:2005-06-20 Published:2005-06-20
Contact: Author for correspondence. E-mail: wangyucheng1029@yahoo.com.cn

摘要/Abstract

摘要： 用差异显示技术研究NaHCO3胁迫下星星草(Puccinellia tenuiflora)基因的表达。经Reverse Northern检测, 获得了7个差异表达的基因片段。其中, 6个为胁迫后诱导表达, 1个为胁迫后抑制表达。序列同源性分析表明, 胁迫诱导表达的6个基因片段中, 1个与钙依赖性蛋白激酶(CDPKs)基因同源性较高,其余5个可能为新序列, 胁迫后抑制表达的基因片段与假定的adaptor蛋白基因同源性较高。本研究为进一步研究星星草的抗盐机理奠定了基础。

关键词: 星星草, 差异显示, NaHCO3胁迫

Abstract: The differential display technique was used to study gene expression of Puccinellia tenuiflora treated with NaHCO3. Seven differentially expressed gene segments were obtained through Reverse Northern detection. Among them, six segments were induced and one was inhibited by NaHCO3. A homologous analysis revealed that one of the six NaHCO3-induced gene segments was homologous to the genes encoding calcium-dependent protein kinases （CDPKs）; the other five might be new sequences. The NaHCO3-inhibited gene segment was homologous to a putative adaptor protein-encoding gene. This research provided basic information for further study of the mechanism on salt resistance in P. tenuiflora.

Key words: Puccinellia tenuiflora, Differential display, NaHCO3 stress

王玉成, 杨传平, 刘桂丰, 姜静. 差异显示技术研究NaHCO3 胁迫下星星草基因表达. 植物学报, 2005, 22(03): 307-312.

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