植物学报 ›› 2005, Vol. 22 ›› Issue (02): 163-168.

• 实验简报 • 上一篇    下一篇

绞股蓝核糖体失活蛋白家族编码基因的5 个cDNA 及其下游非编码区

林毅 谢荔岩 陈国强 吴祖建 谢联辉 林奇英   

  1. (福建农林大学植物病毒研究所,生物农药与化学生物学教育部重点实验室 福州 350002)
  • 收稿日期:2003-10-16 修回日期:2004-06-28 出版日期:2005-04-25 发布日期:2005-04-25
  • 通讯作者: 谢联辉

LIN Yi XIE Li-Yan CHEN Guo-Qiang WU Zu-Jian XIE Lian-Hui LIN Qi-Ying   

  1. (Institute of Plant Virology, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002)
  • Received:2003-10-16 Revised:2004-06-28 Online:2005-04-25 Published:2005-04-25
  • Contact: XIE Lian-Hui

摘要: 通过3''RACE克隆策略获得绞股蓝(Gynostemma pentaphyllum)核糖体失活蛋白(ribosome-inactivating protein,RIP) Gynostemmin的5个cDNA序列gynostemminⅠ~Ⅴ及其下游非编码区(3'' untranslated region, 3''UTR)。它们的编码区长度除gynostemminⅡ为825 bp外,其余均为831 bp。其下游非编码区的长度分别为279、174、170、161和171 bp。在3''UTR中,gynostemminⅠ比另外4个多了两个小的茎环结构和一富含AU的不稳定子元件,其mRNA的稳定性可能因此受到影响。

Abstract: Five cDNA sequences and their 3' untranslated regions (3' UTR) of ribosome-inactivating protein from Gynostemma pentaphyllum were isolated and analyzed. The coding region of gynostemminⅡ is 825 bp in length, and those of the other four are 831 bp long. Their 3' UTRs are 279, 174, 170, 161 and 171 bp in length, respectively. Two small stem-loop and one AU-rich element were found only in the 3' UTR of gynostemmin I.