植物学报 ›› 2004, Vol. 21 ›› Issue (05): 556-558.

• 技术与方法 • 上一篇    下一篇

菊花叶盘片转基因再生体系的优化选择

刘军 赵兰勇 丰震 张美蓉 吴银凤   

  1. 1(山东农业大学林学院 泰安 271018) 2(青岛职业技术学院 青岛 266071)
  • 收稿日期:2003-11-04 修回日期:2003-12-31 出版日期:2004-10-20 发布日期:2004-10-20
  • 通讯作者: 丰震

Optimization Selection of Genetic Transformation Regeneration System from Leaves of Dendranthema morifolium

LIU Jun, ZHAO Lan-Yong, FENG Zhen, ZHANG Mei-Rong, WU Yin-Feng   

  1. 1(College of Forestry, Shandong Agriculture University, Tai’an 271018) 2(Qingdao Vocational and Technical College, Qingdao 266071)
  • Received:2003-11-04 Revised:2003-12-31 Online:2004-10-20 Published:2004-10-20
  • Contact: FENG Zhen

摘要: 以菊花(Dendranthema morifolium)无菌苗叶片为外植体,在芽诱导培养基上直接诱导植株再生,优化选择菊花转基因再生体系。筛选出了能直接诱导芽再生的培养基(MS+6-BA3 mg.L-1+NAA 1 mg.L-1)。茎段在有IBA和NAA的1/2MS培养基上诱导生根。生根的小苗在温室里生长良好。

Abstract: A genetic transfomation regeneration system from aseptic seedling leaves of chrysanthemum (Dendranthema morifolium) was developed. Adventitious shoots were directly induced and regenerated from leaf explants of chrysanthemum, and the medium (MS+6-BA 3 mg.L-1+NAA 1 mg. L-1), which could directly induce shoot regeneration, was used. Shoots were rooted on half-strength MS media with IBA and NAA. The rooted plantlets grew well in greenhouse.