Abstract: A DPPH-Microplate assay were made to determine the free radical scavenging activity of the 80% methanol extracts from the leaves of some subtropical woody plants of Rosacece. The results revealed that all the trees tested have different free radical scavenging capacities, and as the raising of extract concentration and the prolonging of incubation time at 37℃, their free radical scavenging capacities increased. Among all trees tested, Malus melliana (Hand.-Mazz.) Rehd, Kerria japonica (L.) DC., Chaenomeles sinensis (Thouin) Koehne , Malus sieboldii (Regel) Rehd., Malus hupehensis (Pamp.) Rehd., Rosa banksiae Ait., Rosa cymosa Tratt.and Sorbus amabilis Cheng et Yu showed the stronger free radical scavenging capacity, the extracts at a concentration that equals to 0.5 mg leaves per milliliter and after incubating for 20 min, their scavenging rates to 0.5 mmol/L DPPH were 85.0%、75.8%、70.7%、69.6%、64.5%、62.5%、61.8% and 61.3% respectively.