Abstract: Factors affecting the regeneration of rice plants from young panicles , such as developmental stage of young panicles in different genotypes, sterilizing methods, duration of 4℃ treatment, induction, differentiation and rooting conditions, etc., were investigated. The results showed that the optimal conditions for different genotypic rice were as following: after the best developmental stage ( Ⅲ~Ⅳ of indica,Ⅰ~Ⅱ of japonica,Ⅴ of wide compatible variety, WCV) of young panicles was treated at 4℃ within 5 days, the materials were sterilized with 0.5% potassium hypochlorite for 20 minutes, cultivated in YS-Y medium about 20 days to induce the calli , then transferred to YS-YF、YS-F medium about 15 days to regenerate shoots and YS-SG medium for 1~2 weeks to induce the roots. Under the optimal conditions, the frequency of green shoot differentiation was a little more than 85% (indica), 90% (japonica), 85% (WCV), the frequency of root regenerated was about 115% (indica), 130% (japonica), 115% (WCV), respectively. Therefore a high efficient regeneration system from in vitro culture of young panicles of rice was established for genetic transformation of rice.