植物学报 ›› 2003, Vol. 20 ›› Issue (05): 558-564.

• 研究论文 • 上一篇    下一篇

超高产水稻组合‘培矮64S/E32’的耐光氧化特性及其机理

欧志英 彭长连林桂珠 温 学   

  1. (中国科学院华南植物研究所 广州 510650)
  • 收稿日期:2002-01-15 修回日期:2002-11-25 出版日期:2003-10-20 发布日期:2003-10-20
  • 通讯作者: 彭长连

The Characters and Mechanism of Photooxidation Tolerance in Leaves of Peiai64S/E32, a Super High-yielding Hybrid Rice (Oryza sativa L.)

OU Zhi-Ying PENG Chang-LianLIN Gui-Zhu WEN Xue   

  1. (South China Institute of Botany, the Chinese Academy of Sciences, Guangzhou 510650)
  • Received:2002-01-15 Revised:2002-11-25 Online:2003-10-20 Published:2003-10-20
  • Contact: PENG Chang-Lian

摘要: 甲基紫精(MV)介导的光氧化导致两个水稻品种‘汕优63’(目前我国大面积推广的杂交水稻)和‘培矮64S/E32’(新培育的超高产杂交水稻)细胞电解质渗漏率都增加,前者增加的量大于后者,显示‘培矮64S/E32’的细胞膜系统受光氧化的伤害小。光氧化条件下,‘培矮64S/E32’仍能维持较高的光合放氧能力(Amax、Φi)、PSⅡ活性(Fv/Fm、ΦPSⅡ)和叶绿素荧光猝灭系数(qP、NPQ),而且光氧化引起‘培矮64S/E32’这些参数的下降幅度也小。另外,光氧化导致了两个水稻品种抗氧化酶SOD和APX活性增加,‘培矮64S/E32’增加的幅度约为‘汕优63’的3倍。结果表明超高产水稻具有更强的耐光氧化能力。

Abstract: Increase in cell membrane ion leakage was induced by Methyl Viologen-mediated photooxidation in two rice cultivars, ‘Shanyou63'' (developed hybrid cultivar) and ‘Peiai64S/E32'' (super high-yielding hybrid cultivar). More increment of cell membrane leakage was shown in ‘Shanyou63'' than that in ‘Peiai64S/E32'' which means less damage to cell membrane in ‘Peiai64S/E32'' than in ‘Shanyou63'' under photooxidation.‘Peiai64S/E32'' had higher values of photosynthetic oxygen evolution competence (Amax,Φi)、PSⅡ activity (Fv/Fm, ΦPSⅡ) and chlorophyll fluorescence quenching coefficient (qP, NPQ) and less reduction under photooxidation in comparison with ‘Shanyou63''. In addition, ‘Peiai64S/E32'' had as much as 3 folds increase in activities of SOD and APX than ‘Shanyou63'' under photooxidation. These results indicated that super high-yielding rice ‘Peiai64S/E32'' exhibited more resistance to photooxidation mediated by MV than ‘Shanyou63''.