利用花粉管通道法获得转雪花莲凝集素基因（sgna）小麦

植物学报 ›› 2003, Vol. 20 ›› Issue (02): 198-204.

利用花粉管通道法获得转雪花莲凝集素基因（sgna）小麦

侯文胜郭三堆路明

(西北农要科技大学农学院国家小麦改良中心杨凌分中心　陕西杨凌　712100)

收稿日期:2002-05-20 修回日期:2002-06-01 出版日期:2003-04-20 发布日期:2003-04-20
通讯作者: 侯文胜

Development of Transgenic Wheat with Galanthus navies Agglutinin Gene (sgna) via the Pollen-tube Pathway

HOU Wen-ShengGUO San-Dui LU Ming

(Yangling Branch of China Wheat Improvement Center, College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100)

Received:2002-05-20 Revised:2002-06-01 Online:2003-04-20 Published:2003-04-20
Contact: HOU Wen-Sheng

摘要/Abstract

摘要： 利用适用于禾谷类作物的表达载体pGU4AGBar和pGBIU4AGBar，采用花粉管通道法，将人工合成的雪花莲凝集素基因sgna导入了优良冬小麦品系西农2208和西农132，经PCR 和Southern blot鉴定，证明获得了20株导入了sgna基因的转基因植株，转化率约为0.2 8%～0.84%，并通过Western blot鉴定检测到了目的蛋白的表达。

侯文胜郭三堆路明. 利用花粉管通道法获得转雪花莲凝集素基因（sgna）小麦. 植物学报, 2003, 20(02): 198-204.

HOU Wen-ShengGUO San-Dui LU Ming. Development of Transgenic Wheat with Galanthus navies Agglutinin Gene (sgna) via the Pollen-tube Pathway. Chinese Bulletin of Botany, 2003, 20(02): 198-204.

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