植物学报 ›› 1999, Vol. 16 ›› Issue (01): 80-85.

• 技术与方法 • 上一篇    下一篇

植物基因克隆的策略和方法

舒群芳 赵路 李文彬 孙勇如   

  1. 1(中国科学院遗传研究所 北京 100101) 2(首都师范大学化学系 北京 100037)
  • 出版日期:1999-01-20 发布日期:1999-01-20
  • 通讯作者: 舒群芳

Strategies and Methods for Cloning Plant Genes

SHU Qun-Fang, ZHAO Lu, LI Wen-Bin and SUN Yong-Ru   

  1. 1(Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101) 2(The Capital Normal University, Beijing 100037)
  • Online:1999-01-20 Published:1999-01-20
  • Contact: SHU Qun-Fang

摘要: 本文介绍了功能克隆,定位克隆,表型克隆等9种克隆植物基因的方法,着重分析了每项克隆方法的工作原理,应用范围和进展。

Abstract: The strategies for cloning plant genes and the advance were reviewed here. These include functional cloning, positional cloning, phenotype cloning, mRNA differential display, and transposon tagging method.