植物学报 ›› 1998, Vol. 15 ›› Issue (增刊): 119-119.

• 技术与方法 • 上一篇    

DIG系统在Northern杂交中的应用

何煜 陈敏 白书农   

  1. 1(武汉大学 武汉 430072) 2(中国科学院植物研究所 北京 100093)
  • 出版日期:1998-12-20 发布日期:1998-12-20
  • 通讯作者: 何煜

The Application of DIG System to Northern Blot

HE Yu, CHEN Min and BAI Shu-Nong   

  1. 1(Wuhan University, Wuhan 430072) 2(Institute of Botany, Chinese Academy of Sciences, Beijing 100093)
  • Online:1998-12-20 Published:1998-12-20
  • Contact: HE Yu

摘要: 非放射性标记已越来越广泛地被应用于分子牛物学的研究。本文介绍本实验利用DIG高效DNA标记系统进行Northern blot的经验,供同行参考。

Abstract: Non-radioactive labeling technique is now widely accepted as a routine procedure inmolecular biology researches. This report introduced our protocol of using DIG labeled DNA probes in Northern blot, which is sensitive enough to detect the light-regulated expression of spinach rbcL gene.