绿豆子叶脱分化过程中RNA聚合酶的制备、特性及其活性的研究

植物学报 ›› 1991, Vol. 8 ›› Issue (02): 34-38.

绿豆子叶脱分化过程中RNA聚合酶的制备、特性及其活性的研究

徐桂芳张静兰

（中国科学院植物研究所　北京　100044）

出版日期:1991-06-10 发布日期:1991-06-10

Studies on RNA Polymerases Isolation Purification Properties and Activity in Cotyledon Segment of Phaseolus radioutus During Dedifferentiation

Xu Gui-fang and Zhang Jing-lan

(Institute of Botany, Academia Sinica, Beijing 100044)

Online:1991-06-10 Published:1991-06-10

摘要/Abstract

摘要： 绿豆(Phaseolus vadiatus L.)子叶切段在脱分化形成愈伤组织过程中，制备的染色质具有较高的RNA聚合酶活力，3天愈伤组织的酶活力高于5天和7天的；3天、5天和7天加激素脱柱依次分分化形成的愈伤组织，其酶活力均比不脱分化的高一倍以上，用DEAE —纤维素酶部，依据层析洗脱性质和层析图谱及对d—鹅膏菌素的敏感程度，证明三个峰是RNA聚合I，II和III。

Abstract: Chromtin bound DNA-dependent RNA polymerase was isolated from mung bean cotyledon segment during dedifferentiation. The results show that the activity of RNA polymerase in dedifferentiation tissue after culturing for 3 days is higher than that of 5 and 7 days. The activities of RNA polymerase in dedifferentiation tissue for 3, 5 and 7 days are higher than that of control. After fractionation on DEAE-CELLUOSE column, three enzyme peaks were detected in the elute fractions. According to their sensitivity to a-amanitin they are the RNA polymerases I, II and III resp.

徐桂芳张静兰. 绿豆子叶脱分化过程中RNA聚合酶的制备、特性及其活性的研究. 植物学报, 1991, 8(02): 34-38.

Xu Gui-fang and Zhang Jing-lan. Studies on RNA Polymerases Isolation Purification Properties and Activity in Cotyledon Segment of Phaseolus radioutus During Dedifferentiation. Chinese Bulletin of Botany, 1991, 8(02): 34-38.

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