植物学报 ›› 1990, Vol. 7 ›› Issue (04): 37-39.

• 研究报告 • 上一篇    下一篇

不同pH值对樱草原生质分离的影响

娄和林 李世承 崔久满   

  1. 1(辽宁大学生物系,沈阳 110036) 2(辽宁大学科研处)
  • 出版日期:1990-12-10 发布日期:1990-12-10

Effedt of Different PH on Separation of Primula obconica Protoplast

Lou He-lin, Li Shi-cheng and Cui Jiu-man   

  1. 1(Department of Biology, Liaoning University, Shenyang 110036) 2(Scientific Research Centre, Liaoning University)
  • Online:1990-12-10 Published:1990-12-10

摘要: 用国产纤维素酶分离四季樱草叶肉细胞原生质体,分别在八种pH值(5.2,5.4,5.6,5.8,6.0,6.2,6.4和6.6)下进行酶处理试验,得出5.6是最佳pH值的结果。

Abstract: Protoplasts were separated from mesophyll cells of primula obcouica under different pH conditions(5.2, 5.4, 5.6, 5.8, 6.0, 6.2, 6.4 and 6.6)with cellulase(made in China).The results showed that the optimal pH is 5.6.