植物学报 ›› 2015, Vol. 50 ›› Issue (2): 198-205.DOI: 10.3724/SP.J.1259.2015.00198

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水稻散状穗突变体sp的遗传分析及基因初定位

刘丹, 王嘉宇, 刘进, 马殿荣, 赵明辉, 陈温福*()   

  1. 沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室/北方超级粳稻育种教育部重点实验室, 沈阳 110866
  • 收稿日期:2014-04-01 接受日期:2014-05-14 出版日期:2015-03-01 发布日期:2015-04-10
  • 通讯作者: 刘丹,王嘉宇,陈温福
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    辽宁省高等学校优秀人才支持计划(No;LJQ2013075)

Genetic Analysis and Gene Mapping of a Rice Spreadingpanicle Mutant

Dan Liu, Jiayu Wang, Jin Liu, Dianrong Ma, Minghui Zhao, Wenfu Chen*   

  1. Rice Research Institute of Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology, Genetics and Breeding of Ministry of Agriculture/Key Laboratory of Northern Japonica Super Rice Breeding, Ministry ofEducation, Shenyang 110866, China
  • Received:2014-04-01 Accepted:2014-05-14 Online:2015-03-01 Published:2015-04-10
  • Contact: Liu Dan,Wang Jiayu,Chen Wenfu
  • About author:

    ? These authors contributed equally to this paper

摘要:

水稻(Oryza sativa)是我国最主要的粮食作物之一, 其穗部形态直接影响着水稻产量和稻米品质。在秋光和七山占构建的重组自交系群体中发现了1个散穗突变体材料sp (spreading panicle), 田间表现为穗部一次枝梗向外延伸, 与穗轴夹角增大, 且向四周散开, 故暂命名为散穗突变体sp。与野生型相比, 突变体sp穗重、每穗粒重、千粒重、粒宽以及粒厚均极显著减少, 推测SP可能是1个参与调控穗部形态建成和颖花发育的基因。遗传分析表明, 该性状受1个显性核基因控制。利用sp与02428构建的F2群体进行基因定位, 将该基因定位在4号染色体长臂端, 位于E3和RM17578之间的62.9 kb区域内。该结果将为SP基因的图位克隆和揭示其作用机理奠定基础。

Abstract:

Rice is the most important crop in our country, and the panicle traits determine the yield and quality of rice. From recombinant inbred lines derived from a cross between Akihikari (japonica) and Qishanzhan (indica), a spreading- panicle mutant (sp) was found that had panicle branches extending outward, increased angle between primary branch and rachis and the panicle grew around this. As compared with the wild-type parent, sp showed significantly reduced panicle weight, grain weight per panicle, 1 000 grain weight, grain width and grain thickness, which implied that the sp gene participated in regulating panicle formation and glumous flower development. Genetic analysis showed that the sp phenotype was controlled by a single dominance nuclear gene. Primary mapping based on the F2 line between sp and 02428 showed that the sp gene was on the long arm of chromosome 4 and at a 62.9 kb region between markers E3 and RM17578. This information provides a basis for cloning this gene and functional mechanism analysis.