eATP位于H2S下游参与乙烯诱导的拟南芥气孔关闭过程

doi:10.3724/SP.J.1259.2015.00022

植物学报 ›› 2015, Vol. 50 ›› Issue (1): 22-31.DOI: 10.3724/SP.J.1259.2015.00022

eATP位于H₂S下游参与乙烯诱导的拟南芥气孔关闭过程

车永梅^†, 张丹丹^†, 侯丽霞, 王兰香, 刘新^*()

收稿日期:2013-11-06 接受日期:2014-04-03 出版日期:2015-01-01 发布日期:2015-04-09
通讯作者: 车永梅,张丹丹,刘新
作者简介:
? 共同第一作者
基金资助:
国家自然科学基金(No.31170237)、山东省自然科学基金(No.ZR2010CM024)和山东省泰山学者团队建设工程

Extracellular Adenosine Triphosphate Functions Downstream of Hydrogen Sulfide in Ethylene-induced Stomatal Closure in Arabidopsis thaliana

Yongmei Che^†, Dandan Zhang^†, Lixia Hou, Lanxiang Wang, Xin Liu^*

Shandong Key Laboratory of Plant Biotechnology in University, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China

Received:2013-11-06 Accepted:2014-04-03 Online:2015-01-01 Published:2015-04-09
Contact: Che Yongmei,Zhang Dandan,Liu Xin
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 以拟南芥(Arabidopsis thaliana)野生型、H₂S合成突变体(Atl-cdes和Atd-cdes)和ABC转运体突变体(Atmrp4、Atmrp5 和Atmrp4/5)为材料, 探讨乙烯诱导气孔关闭过程中eATP与H₂S之间的关系。结果显示, ABC转运体阻断剂格列本脲(Gli)、P2受体抑制剂磷酸吡哆醛-6-偶氮苯基-2',4'-二硫酸(PPADS)和三磷酸腺苷双磷酸酶(Apyrase)可抑制乙烯诱导的气孔关闭乙烯可提高拟南芥幼苗叶片eATP含量及AtMRP4和AtMRP5相对表达量, 但对Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片eATP含量和气孔运动没有显著作用。实验结果表明, eATP是乙烯诱导拟南芥气孔关闭过程的重要信号分子, AtMRP4和AtMRP5参与胞内ATP的分泌H₂S清除剂次牛磺酸(HT)能阻遏乙烯诱导的拟南芥幼苗叶片eATP含量的升高乙烯对Atl-cdes、Atd-cdes幼苗叶片eATP含量及AtMRP4和AtMRP5相对表达量无显著影响。据此推测eATP位于H₂S下游参与乙烯诱导的拟南芥气孔关闭过程。

Abstract: Using wild-type Arabidopsis, hydrogen sulfide (H₂S)-synthesis mutants (Atl-cdes and Atd-cdes) and ABC- transporter mutants (Atmrp4, Atmrp5 and Atmrp4/5), we examined the effect of extracellular adenosine triphosphate (eATP) on ethylene-induced stomatal closure and its relationship with H₂S. The ABC transporter blocker glibenclamide, P2 receptor inhibiter pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid and apyrase inhibited the inducing effects of ethylene on stomatal closure in Arabidopsis and ethylene-enhanced eATP content and upregulated relative expression of AtMRP4 and AtMRP5 in leaves of wild-type Arabidopsis but had no effect on eATP content or stomatal movement in Atmrp4, Atmrp5 and Atmrp4/5 mutants. Thus, eATP is involved in ethylene-induced stomatal closure, and AtMRP4 and AtMRP5 proteins take part in the secretion of intracellular ATP. The H₂S scavenger hypotaurine inhibited the improving effect of ethylene on eATP content in leaves of wild-type Arabidopsis but had no effect on eATP content or relative expression of AtMRP4 and AtMRP5 in Atl-cdes and Atd-cdes mutants. eATP may be located downstream of H₂S in ethylene-induced stomatal closure in Arabidopsis thaliana.

车永梅, 张丹丹, 侯丽霞, 王兰香, 刘新. eATP位于H₂S下游参与乙烯诱导的拟南芥气孔关闭过程. 植物学报, 2015, 50(1): 22-31.

Yongmei Che, Dandan Zhang, Lixia Hou, Lanxiang Wang, Xin Liu. Extracellular Adenosine Triphosphate Functions Downstream of Hydrogen Sulfide in Ethylene-induced Stomatal Closure in Arabidopsis thaliana. Chinese Bulletin of Botany, 2015, 50(1): 22-31.

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图/表 10

Figure 1 Gli、PPADS和Apyrase对乙烯诱导的拟南芥气孔关闭的影响不同小写字母表示差异显著(P<0.05)

Effects of Gli, PPADS and Apyrase on ethylene induced stomatal closure in leaves of Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)

图2 Gli对乙烯诱导拟南芥幼苗叶片eATP含量的影响

Figure 2 Effects of Gli on ethylene-induced eATP level in seedling leaves of Arabidopsis thaliana

图4 乙烯对拟南芥Atmrp4、Atmrp5和Atmrp4/5叶片气孔开度的影响不同小写字母表示差异显著(P<0.05)

Figure 4 Effects of ethylene on stomatal movement in seedling leaves of Arabidopsis thaliana Atmrp4, Atmrp5 and Atmrp4/5 Different lowercase letters indicated significant difference (P<0.05)

图3 乙烯对拟南芥幼苗叶片AtMRP4和AtMRP5相对表达量的影响不同小写字母表示差异显著(P<0.05)

Figure 3 Effects of ethylene on relative expression of AtMRP4 and AtMRP5 genes in Arabidopsis thaliana seedling leaves Different lowercase letters indicated significant difference (P<0.05)

图5 乙烯对拟南芥Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片eATP含量的影响不同小写字母表示差异显著(P<0.05)

Figure 5 Effects of ethylene on eATP level in seedling leaves of Arabidopsis thaliana Atmrp4, Atmrp5 and Atmrp4/5 Different lowercase letters indicated significant difference (P<0.05)

图6 H2S清除剂HT对乙烯调控拟南芥幼苗叶片eATP含量的影响不同小写字母表示差异显著(P<0.05)

Figure 6 Effects of H2S scavenger HT on eATP level in seedling leaves of Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)

图7 乙烯对拟南芥Atl-cdes和Atd-cdes幼苗叶片eATP含量的影响不同小写字母表示差异显著(P<0.05)

Figure 7 Effects of ethylene on eATP level in seedling leaves of Arabidopsis thaliana Atl-cdes and Atd-cdes Different lowercase letters indicated significant difference (P<0.05)

图8 H2S对拟南芥Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片eATP含量的影响不同小写字母表示差异显著(P<0.05)

Figure 8 Effects of H2S on eATP level in seedling leaves of Atmrp4, Atmrp5 and Atmrp4/5 in Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)

图9 H2S对拟南芥叶片AtMRP4(A)和AtMRP5(B)相对表达量的影响不同小写字母表示差异显著(P<0.05)

Figure 9 Quantitative RT-PCR analysis of H2S-induced AtMRP4 (A) and AtMRP5 (B) relative expression in leaves of Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)

图10 乙烯对拟南芥Atl-cdes (A, B)和Atd-cdes (C, D)叶片AtMRP4和AtMRP5基因表达量的影响不同小写字母表示差异显著(P<0.05)

Figure 10 Effects of ethylene on relative expression of AtMRP4 and AtMRP5 genes in Arabidopsis thaliana leaves of Atl-cdes (A, B) and Atd-cdes (C, D) Different lowercase letters indicated significant difference (P<0.05)

参考文献 28

1	高巍, 尚忠林 (2010). 细胞内离子在气孔运动中的作用. 植物学报 45, 632-639.
2	侯智慧, 车永梅, 王兰香, 侯丽霞, 刘新 (2012). H2S位于H2O2下游参与乙烯诱导拟南芥气孔关闭过程. 植物生理学报 48, 1193-1199.
3	侯智慧, 刘菁, 侯丽霞, 李希东, 刘新 (2011). H2S可能作为H2O2的下游信号介导茉莉酸诱导的蚕豆气孔关闭. 植物学报 46, 396-406.
4	刘国华, 刘菁, 侯丽霞, 唐静, 刘新 (2009). NO可能作为Ca2+的下游信号介导乙烯诱导的蚕豆气孔关闭. 分子细胞生物学报 42, 145-155.
5	Bodin P, Burnstock G (2001). Purinergic signaling: ATP release. Neurochem Res 26, 959-969.
6	Clark G, Darwin C, Mehta V, Jackobs F, Perry T, Hougaard K, Roux S (2013). Effects of chemical inhibitors and apyrase enzyme further document a role for apyrases and extracellular ATP in the opening and closing of stomates in Arabidopsis.Plant Signal Behav 8, e26093.
7	Clark G, Fraley D, Steinebrunner I, Cervantes A, Onyirimba J, Liu A, Torres J, Tang WQ, Kim J, Roux SJ (2011). Extracellular nucleotides and apyrases regulate stomatal aperture in Arabidopsis.Plant Physiol 156, 1740-1753.
8	Duan S, Anderson CM, Keung EC, Chen Y, Chen Y, Swanson RA (2003). P2X7 receptor-mediated release of excitatory amino acids from astrocytes.J Neurosci 23, 1320-1328.
9	Gaedeke N, Klein M, Kolukisaoglu U, Forestier C, Müller A, Ansorge M, Becker D, Mamnun Y, Kuchler K, Schulz B, Mueller-Roeber B, Martinoia E (2001). The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement.EMBO J 20, 1875-1887.
10	García-Mata C, Lamattina L (2010). Hydrogen sulphide, a novel gasotransmitter involved in guard cell signaling.New Phytol 188, 977-984.
11	Hao LH, Wang WX, Chen C, Wang YF, Liu T, Li X, Shang ZL (2012). Extracellular ATP promotes stomatal opening of Arabidopsis thaliana through heterotrimeric G protein α subunit and reactive oxygen species.Mol Plant 5, 852-864.
12	He JM, Yue XZ, Wang RB, Zhang Y (2011). Ethylene mediates UV-B-induced stomatal closure via peroxidase-dependent hydrogen peroxide synthesis in Vicia faba L.J Exp Bot 62, 2657-2666.
13	Iqbal N, Nazar R, Syeed S, Masood A, Khan NA (2011). Exogenously-sourced ethylene increases stomatal conductance, photosynthesis, and growth under optimal and deficient nitrogen fertilization in mustard.J Exp Bot 62, 4955-4963.
14	Jeter CR, Tang WQ, Henaff E, Butterfield T, Roux SJ (2004). Evidence of a novel cell signaling role for extracellular adenosine triphosphates and diphosphates in Arabidopsis.Plant Cell 16, 2652-2664.
15	Kim SY, Sivaguru M, Stacey G (2006). Extracellular ATP in plants, visualization, localization, and analysis of physiological significance in growth and signaling.Plant Physiol 142, 984-992.
16	Klein M, Geisler M, Suh SJ, Kolukisaoglu HÜ, Azevedo L, Plaza S, Curtis MD, Richter A, Weder B, Schulz B, Martinoia E (2004). Disruption of AtMRP4, a guard cell plasma membrane ABCC-type ABC transporter, leads to deregulation of stomatal opening and increased drought susceptibility.Plant J 39, 219-236.
17	Klein M, Perfus-Barbeoch L, Frelet A, Gaedeke N, Reinhardt D, Mueller-Roeber B, Martinoia E, Forestier C (2003). The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signaling and water use.Plant J 33, 119-129.
18	Liu J, Liu GH, Hou LX, Liu X (2010). Ethylene-induced nitric oxide production and stomatal closure in Arabidopsis thaliana depending on changes in cytosolic pH.Chinese Sci Bull 55, 2403-2409.
19	Nagy R, Grob H, Weder B, Green P, Klein M, Frelet- Barrand A, Schjoerring JK, Brearley C, Martinoia E (2009). The Arabidopsis ATP-binding cassette protein AtMRP5/AtABCC5 is a high affinity inositol hexakisphosphate transporter involved in guard cell signaling and phytate storage.J Biol Chem 284, 33614-33622.
20	Raghavendra AS (1981). Energy supply for stomatal opening in epidermal strips of Commelina benghalensis.Plant Physiol 67, 385-387.
21	Suh SJ, Wang YF, Frelet A, Leonhardt N, Klein M, Forestier C, Mueller-Roeber B, Cho MH, Martinoia E, Schroeder JI (2007). The ATP binding cassette transporter AtMRP5 modulates anion and calcium channel activities in Arabidopsis guard cells.J Biol Chem 282, 1916-1924.
22	Sun J, Zhang CL, Deng SR, Lu CF, Shen X, Zhou XY, Zheng XJ, Hu ZM, Chen SL (2012a). An ATP signaling pathway in plant cells: extracellular ATP triggers programmed cell death in Populus euphratica.Plant Cell Environ 35, 893-916.
23	Sun J, Zhang X, Deng SR, Zhang CL, Wang MJ, Ding MQ, Zhao R, Shen X, Zhou XY, Lu CF, Chen SL (2012b). Extracellular ATP signaling is mediated by H2O2 and cytosolic Ca2+ in the salt response of Populus euphratica cells.PLoS One 7, e53136.
24	Thomas C, Rajagopal A, Windsor B, Dudler R, Lloyd A, Roux SJ (2000). A role for ectophosphatase in xenobiotic resistance.Plant Cell 12, 519-533.
25	Tonón C, Cecilia Terrile M, José Iglesias M, Lamattina L, Casalongué C (2010). Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings.J Plant Physiol 167, 540-546.
26	Weerasinghe RR, Swanson SJ, Okada SF, Garrett MB, Kim SY, Stacey G, Boucher RC, Gilroy S, Jones AM (2009). Touch induces ATP release in Arabidopsis roots that is modulated by the heterotrimeric G-protein complex.FEBS Lett 583, 2521-2526.
27	Wilkinson S, Davies WJ (2009). Ozone suppresses soil drying- and abscisic acid (ABA)-induced stomatal closure via an ethylene-dependent mechanism.Plant Cell Environ 32, 949-959.
28	Wu SJ, Liu YS, Wu JY (2008). The signaling role of extracellular ATP and its dependence on Ca2+ flux in elicitation of Salvia miltiorrhiza hairy root cultures.Plant Cell Physiol 49, 617-624.

eATP位于H₂S下游参与乙烯诱导的拟南芥气孔关闭过程

Extracellular Adenosine Triphosphate Functions Downstream of Hydrogen Sulfide in Ethylene-induced Stomatal Closure in Arabidopsis thaliana

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