拟南芥叶绿体分裂突变体cpd111的基因定位与分析

doi:10.3724/SP.J.1259.2012.00226

植物学报

拟南芥叶绿体分裂突变体cpd111的基因定位与分析

刘霞¹, 高岳芳¹, 石玉红¹, 沈鑫曌¹, 刘忠华^1*, 高宏波^1,2*

¹北京林业大学生命科学与技术学院, 北京 100083;
²北京林业大学林木育种国家工程实验室, 北京 100083

收稿日期:2012-01-19 修回日期:2012-03-07 出版日期:2012-05-01 发布日期:2012-07-06
通讯作者: 刘忠华;高宏波

Genetic Mapping and Analysis of a Chloroplast Division Mutant cpd111 in Arabidopsis thaliana

Xia Liu¹, Yuefang Gao¹, Yuhong Shi¹, Xinzhao Shen¹, Zhonghua Liu^1*, Hongbo Gao^1,2*

¹College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

²National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China

Received:2012-01-19 Revised:2012-03-07 Online:2012-05-01 Published:2012-07-06
Contact: Zhonghua Liu;Hongbo Gao

摘要/Abstract

摘要： 通过EMS(ethyl methane sulphonate)诱变从拟南芥(Arabidopsis thaliana)突变体库中筛选到一个叶绿体分裂突变体chloroplast division111 (cpd111)。遗传学分析表明, 该突变体的表型是单基因控制的隐性性状。与野生型相比, 突变体植物细胞的叶绿体数量少, 叶绿体形态和大小多样化, 并且细胞体积与叶绿体数量之间无相关性。利用图位克隆的方法确定cpd111的突变基因为FtsZ1。进一步的分析表明, 该突变影响FtsZ1基因mRNA的正常剪切和稳定性, 使蛋白质翻译提前终止, 最终导致叶绿体分裂异常。该工作为研究FtsZ1在叶绿体分裂中的作用提供了新的材料和线索。

Abstract: Ethyl-methane sulfonate mutagenesis revealed a chloroplast division mutant, chloroplast division111 (cpd111), in Arabidopsis thaliana. Genetic analysis indicated that the mutant was controlled by a single recessive gene. Chloroplasts in the mutant were fewer in number and more heterogeneous in size than those in the wild type. The correlation between chloroplast number and cell size was not obvious in the mutant. Map-based cloning revealed a mutation in FtsZ1 responsible for the mutant phenotype. The mutation affected the splicing and stability of FtsZ1 mRNA, caused early translational termination of the protein and resulted in a chloroplast division defect in cpd111. We provide new materials and information for a better understanding of the function of FtsZ1 in chloroplast division.

刘霞, 高岳芳, 石玉红, 沈鑫曌, 刘忠华, 高宏波. 拟南芥叶绿体分裂突变体cpd111的基因定位与分析. 植物学报, DOI: 10.3724/SP.J.1259.2012.00226.

Xia Liu, Yuefang Gao, Yuhong Shi, Xinzhao Shen, Zhonghua Liu, Hongbo Gao. Genetic Mapping and Analysis of a Chloroplast Division Mutant cpd111 in Arabidopsis thaliana. Chinese Bulletin of Botany, DOI: 10.3724/SP.J.1259.2012.00226.

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拟南芥叶绿体分裂突变体cpd111的基因定位与分析

Genetic Mapping and Analysis of a Chloroplast Division Mutant cpd111 in Arabidopsis thaliana

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