植物学报 ›› 2011, Vol. 46 ›› Issue (6): 642-651.DOI: 10.3724/SP.J.1259.2011.00642

• 研究报告 • 上一篇    下一篇

美洲黑杨材性相关候选基因PdCYTOB的功能鉴定

李少锋, 苏晓华*, 张冰玉, 黄秦军, 褚延广, 丁昌俊   

  1. 中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091
  • 收稿日期:2011-05-04 修回日期:2011-07-11 出版日期:2011-11-01 发布日期:2011-11-18
  • 通讯作者: 苏晓华
  • 基金资助:

    林业公益性行业科研专项“杨树产业资源材培育及新产品开发关键技术研究”;“863”国家高技术项目“杨树分子育种与品种创制”

Functional Identification of Wood-property Candidate Gene PdCYTOB in Populus deltoides

Shaofeng Li, Xiaohua Su*, Bingyu Zhang, Qinjun Huang, Yanguang Chu, Changjun Ding   

  1. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
  • Received:2011-05-04 Revised:2011-07-11 Online:2011-11-01 Published:2011-11-18
  • Contact: Xiaohua Su

摘要: 利用RT-PCR技术研究了美洲黑杨(Populus deltoides)材性相关候选基因细胞分裂素结合蛋白(cytokinin binding protein)基因PdCYTOB的表达谱, 结果显示, 在未成熟木质部、未成熟韧皮部和韧皮部中PdCYTOB基因具有较高水平的表达量。对导入反义PdCYTOB的山新杨(Populus davidiana × P. bolleana)植株进行Southern杂交和RT-PCR检测, 证实反义PdCYTOB基因已整合到杨树基因组中并表达。对大田转基因株系及对照植株进行表型观察、组织切片和微纤丝角的测定, 结果表明, 转反义PdCYTOB基因植株的高度明显增加, 木质部、韧皮部变宽, 微纤丝角明显变小, 初步表明转基因杨树在造纸性能上有所改良。这些研究结果对于阐明PdCYTOB在美洲黑杨木材形成中的分子作用机制具有重要的理论意义。

Abstract: We used quantitative real-time RT-PCR to investigate the tissue expression patterns of cytokinin bindingprotein PdCYTOB, which is related to the wood properties of Populus deltoides. PdCYTOB was expressed predominantly in immature xylem, immature phloem and the phloem zone of the stem of P. deltoides. Southern blot and RT-PCR were used to detect antisense PdCYTOB-transgenic Populus davidiana × P. bolleana plants. The antisense PdCYTOB gene was integrated into the genome of the poplar and expressed. Phenotype, anatomical studies and microfibrillar angle analysis revealed the plant height of transgenic poplar higher than that of untransformed lines, as well as xylem and the phloem zone of plants with antisense PdCYTOB wider than those of untransformed lines. In addition, the microfibrillar angle of transgenic plants was decreased in the test field. Thus, pulping and papermaking properties might be improved in antisense-PdCYTOB transformed lines. Our study sheds light on the molecular mechanism of PdCYTOB in wood formation in P. deltoides.

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