植物学报 ›› 2011, Vol. 46 ›› Issue (6): 632-641.DOI: 10.3724/SP.J.1259.2011.00632

• 研究报告 • 上一篇    下一篇

利用染色体单片段代换系定位水稻株高QTL

朱金燕1, 嵇朝球3, 周勇2, 王军1, 王中德2, 杨杰1, 范方军1, 梁国华2*, 仲维功1*   

  1. 1江苏省农业科学院粮食作物研究所, 国家水稻改良中心南京分中心, 南京 210014
    2扬州大学教育部植物功能基因组学重点实验室, 扬州 225009;
    3江苏油田直属农场, 洪泽 211706
  • 收稿日期:2011-07-20 修回日期:2011-09-21 出版日期:2011-11-01 发布日期:2011-11-18
  • 通讯作者: 梁国华,仲维功

Identification of Plant Height Quantitative Trait Loci with Single Segment Substituted Lines in Rice

Jinyan Zhu1, Chaoqiu Ji3, Yong Zhou2, Jun Wang1, Zhongde Wang2, Jie Yang1, Fangjun Fan1, Guohua Liang2*, Weigong Zhong1*   

  1. 1Nanjing Branch of Chinese National Center for Rice Improvement, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

    2Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China

    3Jiangsu Oilfield Farm, Hongze 211706, China
  • Received:2011-07-20 Revised:2011-09-21 Online:2011-11-01 Published:2011-11-18
  • Contact: Guohua Liang,Weigong Zhong

摘要: 株高是典型的数量性状, 易受遗传背景的干扰和环境因素的影响, 利用单片段代换系(single segment substitution lines, SSSLs)能减少遗传背景的干扰。以85个单片段代换系为材料, 其受体亲本为籼稻广陆矮4号(Oryza sativa ssp. indica), 供体亲本为粳稻日本晴(Oryza sativa ssp. japonica)。通过单因素方差分析和Dunnett’s多重比较, 分析单片段代换系与受体亲本之间株高的差异, 对代换片段上的株高QTL进行鉴定。以P≤0.001为阈值共检测到24个株高QTLs, 分别分布于除第10染色体外的其它11条染色体上, 其中3个QTLs的加性效应表现为减效作用, 另外21个株高QTLs的加性效应表现为增效作用。QTLs加性效应变化范围为–6.5–31.74, 加性效应百分率的变化范围为–8.81%–41.96%。该研究对进一步发掘和利用新的矮秆或半矮秆基因资源具有重要意义。

Abstract: Plant height is a typical quantitative trait and can easily be influenced by genetic background and environmental factors. Single-segment-substitution lines (SSSLs) can effectively reduce the interference of the genetic background. We developed a set of genome-wide SSSLs carrying Nipponbare introgression segments in a background of Guangluai 4. Quantitative trail loci (QTL) analysis involved one-way ANOVA and Dunnett’s test for comparing SSSLs and the recurrent parent Guangluai 4 (P≤0.001). We detected 24 QTLs for plant height distributed on 11 rice chromosomes, except chromosome 10, of which 3 had a negative effect and the others a positive effect. The additive effect of these QTLs ranged from –6.5 to 31.74, with additive effect percentages –8.81% to 41.96%. These results provide useful information for exploring and using new dwarf or semi-dwarf resources.