植物学报

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空间转录组学技术及其在植物研究中的新进展

戴雪妍1, 2, 张正海2, 曹亚从2, 苏强2, 吴华茂1, 2*, 王立浩1, 2*   

  1. 1三亚中国农业科学院国家南繁研究院, 海南省种业实验室, 三亚 572024; 2中国农业科学院蔬菜花卉研究所, 蔬菜生物育种全国重点实验室, 北京 100081


  • 收稿日期:2025-10-29 修回日期:2025-12-05 出版日期:2025-12-24 发布日期:2025-12-24
  • 通讯作者: 吴华茂, 王立浩
  • 基金资助:

    中国农业科学院科技创新工程基础科学研究中心(No.CAAS-BRC-HS-2025-01)、三亚中国农业科学院国家南繁研究院“南繁专项一般项目(No.YBXM2418, No.YBXM2522)、海南省种业实验室与中国种子集团有限公司联合揭榜挂帅项目(No.B23CQ15KP)、中国农业科学院科技创新工程(No.Y2024QC06)、国家自然科学基金青年科学基金(No.32302557)、国家重点研发计划(No.2023YFD1200101)、国家自然科学基金面上项目(No.32372712)、国家自然科学基金重大项目(No.32494780)、中国农业科学院科技创新工程(No.CAASASTIP-IVFCAAS)、现代农业产业技术体系建设专项(No.CARS-23-A15)和农业农村部园艺作物生物学与种质创制(蔬菜)重点实验室

Spatial Transcriptomics Techniques and Its New Advances in Plant Research

Xueyan Dai1, 2, Zhenghai Zhang2, Yacong Cao2, Qiang Su2, Huamao Wu1, 2*, Lihao Wang1, 2*   

  1. 1Sanya National Nanfan Research Institute of the Chinese Academy of Agricultural Sciences, Hainan Yazhou Bay Seed Industry Lab, Sanya 572024, China; 2Institute of Vegetables & Flowers, Chinese Academy of Agricultural Sciences, State Key Laboratory of Vegetable Biobreeding, Beijing 100081, China

  • Received:2025-10-29 Revised:2025-12-05 Online:2025-12-24 Published:2025-12-24
  • Contact: Huamao Wu, Lihao Wang
  • Supported by:
    Innovation Program of Chinese Academy of Agricultural Sciences;Nanfan special project, Chinese Academy of Agricultural Sciences;the National Natural Science Foundation of China for Youth Scholar;the Agricultural Science and Technology Innovation Program (ASTIP);China Agriculture Research System;Hainan Seed Industry Laboratory and China National Seed Group;the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences;the Major Project of the National Natural Science Foundation of China

摘要: 空间转录组学是获取并解析转录组空间位置信息的重要工具。近年来, 随着分辨率、通量和灵敏度的提升, 该技术在生命科学领域取得了显著进展。目前, 空间转录组学在生物、医药和植物学等多个领域得到广泛应用。该文回顾了空间转录组学发展历程, 介绍了主流技术、技术选择策略及相关实验流程, 概述了空间转录组学在细胞类型识别与功能解析、植物生长发育、抗逆机制及作物育种应用中取得的进展。此外, 文章还从空间转录组学视角进行跨物种比较, 提出了目前技术发展中存在分辨率与通量平衡、植物组织的特殊性、数据分析和标准化等方面的瓶颈问题。最后, 展望了未来空间转录组学在技术创新、辅助分子生物育种, 以及数据整合与平台构建等中的潜在研究方向, 旨在为相关领域的研究提供参考借鉴。

关键词: 空间转录组学, 发展历程, 植物研究, 应用进展

Abstract: Spatial transcriptomics is an important tool for obtaining and analyzing the spatial location information of the transcriptome. In recent years, with the improvement of resolution, throughput and sensitivity, this technology has made significant progress in the field of life sciences. Currently, spatial transcriptomics is widely applied in multiple fields such as biology, medicine and botany. This article reviews the development history of spatial transcriptomics, introduces mainstream technologies, technical selection strategies and related experimental procedures, and summarizes the progress made in the application of spatial transcriptomics in cell type identification and functional analysis, plant growth and development, stress resistance mechanisms and crop breeding. In addition, the article conducts cross-species comparisons from the perspective of spatial transcriptomics, and points out the bottleneck problems existing in the current technological development, such as the balance between resolution and throughput, the particularity of plant tissues, data analysis and standardization. Finally, it looks forward to potential research directions in the future, including technological innovation, assisting molecular biological breeding, and data integration and platform construction, aiming to provide reference and inspiration for related research fields.

Key words: spatial transcriptomics, developmental history, plant research, application progress