伴矿景天SpMTP10介导锰积累的机制

doi:10.11983/CBB25053

植物学报

伴矿景天SpMTP10介导锰积累的机制

陈思颖1,2†, 王晶琳1†, 李颖怡1, 卢湘鑫1, 张培红1, 邱庆红1, 高艳1,2, 顾天宇1,2, 彭佳师1, 2*

1湖南科技大学生命科学与健康学院, 湘潭411201; 2经济作物遗传改良与综合利用湖南省重点实验室/重金属污染土壤生态修复与安全利用湖南省高校重点实验室, 湘潭 411201

收稿日期:2025-04-02 修回日期:2025-06-10 出版日期:2025-09-03 发布日期:2025-09-03
通讯作者: 彭佳师
基金资助:
湖南省自然科学基金(No.2024JJ6213), 湖南省教育厅科学研究项目(No.23A0372, No.24A0352), 大学生创新创业训练计划项目(No.S202410534051)

The Mechanism of Manganese Accumulation Mediated by SpMTP10 Isolated from Sedum plumbizincicola

Siying Chen1, 2†, Jinglin Wang1†, Yingyi Li1, Xiangxin Lu1, Peihong Zhang1, Qinghong Qiu1, Yan Gao1,2, Tianyu Gu1,2, Jiashi Peng1, 2*

1School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, China; 2Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Xiangtan 411201, China

Received:2025-04-02 Revised:2025-06-10 Online:2025-09-03 Published:2025-09-03
Contact: Jiashi Peng

摘要/Abstract

摘要： 锰(Mn)是植物必需矿质元素, 其缺乏或过量都会影响植物的生长发育。鉴定植物中介导Mn积累的关键基因并揭示其作用机制, 对于相关作物遗传改良以及Mn污染生物修复都具有重要意义。本研究从超积累植物伴矿景天(Sedum plumbizincicola)中克隆了一个阳离子扩散促进子(CDF, cation diffusion facilitator)家族成员基因SpMTP10 (metal tolerance protein 10)。其在酵母中表达后能够极大地增强转化子对过量Mn胁迫的耐受性, 同时转化子中Mn的积累增加, 但是对镉(Cd)、锌(Cd)、铜(Cu)、铁(Fe)等其他金属元素过量胁迫的耐受性以及积累的作用不明显。亚细胞定位分析表明其定位于内质网膜。在拟南芥中异源表达SpMTP10使植物根部Mn积累减少, 地上部Mn积累增多, 且对过量Mn胁迫更加敏感。综上所述, SpMTP10可能通过促进Mn向内质网的区隔增加酵母对过量Mn毒害的耐受性; 而在植物中表达后, SpMTP10介导的Mn向内质网的转运则可能增加了内质网腔中的Mn通过胞间连丝进行在细胞间的迁移, 从而在促进根部的Mn向维管组织的装载并往地上部转运的过程中发挥作用。

关键词: 锰, 内质网, 金属耐受蛋白, 伴矿景天

Abstract: INTRODUCTION: Manganese (Mn) is an essential micronutrient for plant growth and primarily act as enzyme cofactors and participate in the redox processes. However, excessive absorption of Mn by plant can also induce toxicity damages. Therefore, plants need to tightly regulate the uptake, homeostasis, and distribution of Mn to cope with stresses caused by its deficiency or excess. In these processes, the cation diffusion facilitator (CDF) family transporters, which in plants are also known as metal tolerance proteins (MTP), had been shown to be crucial for Mn homeostasis. Therefore, identifying MTP family genes and elucidating their underlying mechanisms for Mn accumulation would not only provide the novel insights about basic scientific issues of plant Mn accumulation, but also gene resources for crops improvement and Mn pollution bioremediation. RATIONALE: Sedum plumbizincicola is a recently discovered Cd/Zn hyperaccumulator that grows in mining areas. The soil in its natural habitat contains more than 10 000 mg·kg-1 of Mn, suggesting that S. plumbizincicola may have efficient Mn transport and detoxification capabilities. Based on the transcriptome sequencing results of S. plumbizincicola obtained previously, a member of the MTP family gene named SpMTP10 was cloned and its role in mediating Mn accumulation was investigated in this study. RESULTS: Phylogenetic analysis with orthologs from Arabidopsis and rice revealed that SpMTP10 belongs to the Mn-CDF subfamily and is most closely related to AtMTP10, AtMTP9 and OsMTP9, with the highest sequence identity of 72% to AtMTP10. SpMTP10 is mainly expressed in the roots of S. plumbizincicola and its expression level is not affected by Mn treatment. Expression of SpMTP10 in yeast can greatly enhance the tolerance of transformants to excessive Mn stress, and increase the Mn accumulation in transformants. However, under conditions of excessive cadmium (Cd), zinc (Zn), copper (Cu), and iron (Fe) stress, the yeast transformants exhibited no significant changes in tolerance. Subsequent subcellular localization analysis revealed that SpMTP10 was localized to the endoplasmic reticulum (ER) membrane. Compared with wild-type plants, transgenic Arabidopsis overexpressing SpMTP10 demonstrated reduced Mn accumulation in roots but increased Mn accumulation in shoots, rendering the plants more sensitive to excessive Mn stress. CONCLUSION: In conclusion, SpMTP10 likely enhances yeast tolerance to excessive Mn toxicity by promoting Mn sequestration in the ER. In plants, Mn transport mediated by SpMTP10 into the ER may facilitate intercellular migration of Mn in the ER lumen via plasmodesmata, thereby promoting Mn movement toward vascular tissues in roots and subsequent long-distance transport to shoots.

Key words: manganese, endoplasmic reticulum, MTP, Sedum plumbizincicola

陈思颖, 王晶琳, 李颖怡, 卢湘鑫, 张培红, 邱庆红, 高艳, 顾天宇, 彭佳师. 伴矿景天SpMTP10介导锰积累的机制. 植物学报, DOI: 10.11983/CBB25053.

Siying Chen, Jinglin Wang, Yingyi Li, Xiangxin Lu, Peihong Zhang, Qinghong Qiu, Yan Gao, Tianyu Gu, Jiashi Peng. The Mechanism of Manganese Accumulation Mediated by SpMTP10 Isolated from Sedum plumbizincicola. Chinese Bulletin of Botany, DOI: 10.11983/CBB25053.

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伴矿景天SpMTP10介导锰积累的机制

The Mechanism of Manganese Accumulation Mediated by SpMTP10 Isolated from Sedum plumbizincicola

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