适配体技术及其在植物科学研究中的应用

doi:10.11983/CBB23050

植物学报 ›› 2023, Vol. 58 ›› Issue (6): 935-945.DOI: 10.11983/CBB23050

适配体技术及其在植物科学研究中的应用

李奕¹^,²^,³^,^†, 张曦¹^,²^,³^,^†, 袁艳辉¹^,²^,³, 公丕昌⁴^,^*(), 林金星¹^,²^,³^,^*()

¹北京林业大学生物科学与技术学院, 林木遗传育种全国重点实验室, 北京 100083
²北京林业大学生物科学与技术学院, 北京 100083
³北京林业大学树木发育与基因编辑研究院, 北京 100083
⁴中国科学院植物研究所, 植物多样性与特色经济作物全国重点实验室, 北京 100093

收稿日期:2023-04-14 接受日期:2023-08-19 出版日期:2023-11-01 发布日期:2023-11-27
通讯作者: * E-mail: gongpeichang@ibcas.ac.cn;linjx@ibcas.ac.cn
作者简介:^† 共同第一作者
基金资助:
中央高校基本科研业务费专项资金(BLX202116);中国科学院青年创新促进会(2019081);国家自然科学基金(32030010);国家自然科学基金(32000558);北京市自然科学基金(5232016);抗松材线虫病高产油松新品种设计与培育(2022ZD0401605)

Aptamers and Their Applications in Plant Science Researches

Yi Li¹^,²^,³^,^†, Xi Zhang¹^,²^,³^,^†, Yanhui Yuan¹^,²^,³, Pichang Gong⁴^,^*(), Jinxing Lin¹^,²^,³^,^*()

¹State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
²College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
³Institute of Tree and Genome Editing, Beijing Forestry University, Beijing 100083, China
⁴State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2023-04-14 Accepted:2023-08-19 Online:2023-11-01 Published:2023-11-27
Contact: * E-mail: gongpeichang@ibcas.ac.cn;linjx@ibcas.ac.cn
About author:^† These authors contributed equally to this paper

摘要/Abstract

摘要： 适配体是一种从人工合成的文库中筛选出来、能够特异结合靶标分子的单链寡核苷酸或多肽。适配体基于自身结构和序列与靶分子特异结合并调节其活性, 应用于生物体内分子功能研究及新型药物制剂的研发。近年来, 多肽适配体广泛应用于医学、遗传学和分子生物学等多个领域, 成为一种高效、特异且功能强大的新工具。在植物研究中, 随着相应体系的建立、应用和推广, 适配体技术逐渐成为研究植物分子功能的有效工具。该文综述了不同类型的适配体、筛选原理及优缺点, 以及在植物研究中的应用。可以预见, 随着分子设计育种技术的发展, 适配体技术有望成为植物科学领域有价值的应用工具。

关键词: 适配体, 核酸, 多肽, 植物, 筛选

Abstract: Aptamers are single-stranded oligonucleotides or short peptides that are screened and selected from artificially synthesized libraries and can specifically bind to the target molecules. Aptamers can bind and regulate the activities of its target molecules based on their own structure and sequence, and are applied to in vivo molecular function researches and new drug preparation explorations. In recent years, peptide aptamers have been widely used in various fields, such as medicine, genetics and molecular biology, which is becoming a highly efficient, specific and powerful new tool. In plants, with the establishment, application and promotion of the corresponding system, aptamers have gradually been become an effective tool for studying plant molecular functions. In this review, we summarized the classification, screening principles and basic applications of aptamers, with special emphasis on their applications in plant breeding. It can be expected that with the development of molecular design bio-breeding technology, aptamers can be cooperated with transgenic techniques and become a valuable application tool in the plant science fields.

Key words: aptamer, nucleic acid, peptide, plants, screening

李奕, 张曦, 袁艳辉, 公丕昌, 林金星. 适配体技术及其在植物科学研究中的应用. 植物学报, 2023, 58(6): 935-945.

Yi Li, Xi Zhang, Yanhui Yuan, Pichang Gong, Jinxing Lin. Aptamers and Their Applications in Plant Science Researches. Chinese Bulletin of Botany, 2023, 58(6): 935-945.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB23050

https://www.chinbullbotany.com/CN/Y2023/V58/I6/935

图/表 2

图1 适配体的分类、筛选及其作用靶标适配体分为核酸适配体和多肽适配体两类。核酸适配体包括DNA适配体和RNA适配体, 筛选方式主要包括配体指数富集系统进化(SELEX)技术以及基于SELEX的微流控芯片技术、镜像筛选技术和计算机建模筛选等。多肽适配体的筛选方式主要包括噬菌体展示技术、化学合成随机多肽库筛选和分子对接模拟筛选等。适配体的靶标主要包括细胞、细菌、病毒、DNA、RNA和蛋白质等。

Figure 1 The classification, screening and targets of aptamers The aptamers can be divided into nucleic acid aptamers and peptide aptamers. The nucleic acid aptamers include DNA aptamers and RNA aptamers, and the screening methods mainly include systematic evolution of ligands by exponential enrichment (SELEX) technology, and SELEX based microfluidic chip, mirror-image and computer modeling. Peptide aptamers can be screened by phage display, random peptide library and molecular docking. The targets of aptamers are mainly involved in cells, bacteria, virus, DNA, RNA, and proteins.

图2 通过人工合成多肽适配体库筛选靶蛋白最适多肽适配体流程利用人工合成肽库的方式, 体外筛选得到与靶蛋白结合的最适多肽适配体。在体外向植物表面喷施多肽适配体, 多肽适配体进入植物细胞, 影响靶蛋白与其它蛋白的相互作用, 从而抑制靶蛋白的功能。

Figure 2 The process of selecting the most suitable peptide aptamer for the target protein through the synthetic peptide aptamer library The optimal peptide aptamer binding to the target protein was screened in vitro by using a synthetic peptide library. Peptide aptamers are sprayed on the surface of plants in vitro, and peptide aptamers enter plant cells to affect the interaction between target proteins and other proteins, thereby inhibiting the function of target proteins.

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适配体技术及其在植物科学研究中的应用

Aptamers and Their Applications in Plant Science Researches

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