植物学报 ›› 2021, Vol. 56 ›› Issue (5): 594-604.DOI: 10.11983/CBB21052

• 技术方法 • 上一篇    下一篇

P515的测量原理、方法和应用

张春艳1,2,*(), 庞肖杰2   

  1. 1中国科学院植物研究所公共技术中心, 北京 100093
    2中国科学院光生物学重点实验室, 北京 100093
  • 收稿日期:2021-03-23 接受日期:2021-06-18 出版日期:2021-09-01 发布日期:2021-08-31
  • 通讯作者: 张春艳
  • 作者简介:*E-mail: zhangchunyan@ibcas.ac.cn
  • 基金资助:
    中国科学院仪器设备功能开发技术创新项目(2018g0048)

The Measurement Principles, Methods and Applications of P515

Chunyan Zhang1,2,*(), Xiaojie Pang2   

  1. 1Plant Science Facility of Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2Key Laboratory of Photobiology, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2021-03-23 Accepted:2021-06-18 Online:2021-09-01 Published:2021-08-31
  • Contact: Chunyan Zhang

摘要:

光谱技术已广泛应用于光合研究领域, 如光吸收信号P515和P700氧化还原动力学以及叶绿素荧光等, 可快速、准确地检测植物的光合活性。P515信号广泛存在于高等植物和藻类中, 是类囊体膜上的色素分子吸收光能后, 其吸收光谱发生位移造成。利用光诱导的P515快速和慢速动力学, 可检测PSI和PSII反应中心的比值、ATP合酶的质子传导性、围绕PSI的环式电子传递速率、质子动力势及其组分, 还可通过同步检测叶绿素荧光和P515信号研究光保护机制。该文总结了P515的主要测量原理、方法及其应用, 旨在为深入研究光合作用机理提供技术支持。

关键词: P515, ATP合酶, 环式电子传递, 质子动力势

Abstract:

The spectral techniques have been widely used in the field of photosynthesis research, such as the light absorption signals P515 and P700 redox kinetics, and chlorophyll fluorescence, which can detect the photosynthetic activities of plants quickly and accurately. P515 signal is widely present in higher plants and algae, which is caused by the shift of absorption spectrum of pigments on thylakoid membrane. We can detect the ratio of PSI to PSII reaction center, the proton conductivity of chloroplast ATP synthase, the cyclic electron flow rate around PSI, the proton motive force (pmf) and its components by the P515 fast and slow kinetics, and study the photoprotective mechanism by simultaneous detection of P515 signal and chlorophyll fluorescence. In this paper, we summarize the main measurement methods of P515, expound its principles, and the applications. The aim is to provide technical supports for further study on the mechanism of photosynthesis.

Key words: P515, ATP synthase, cyclic electron transport, proton motive force