基于多个叶绿体基因序列片段重建广义苋科系统发育关系

doi:10.11983/CBB19228

摘要/Abstract

摘要：

苋科(Amaranthaceae sensu lato)是石竹目(Caryophyllales)第二大科, 目前被普遍接受的苋科为其广义概念, 含狭义苋科(Amaranthaceae sensu stricto)和藜科(Chenopodiaceae)。然而到目前为止, 藜科是否应作为独立的科还存在争议。此外, 广义苋科内部各亚科之间的系统关系也尚未厘清。对广义苋科所有13个亚科代表类群进行取样(共59种), 基于8个叶绿体序列片段重建其系统发育关系, 并结合分子钟估算, 对该科及其主要分支的起源与分化时间进行推测。结果表明, 广义苋科与狭义苋科都是很好的单系, 但藜科并非单系, 因此不支持藜科在科级水平的地位, 支持广义苋科的观点。除了多节草亚科(Polycnemoideae)之外, 其它亚科的系统位置均得到很好的分辨。分子钟估算结果表明, 广义苋科于白垩纪晚期约69.9 Ma分化出该科的2个主要分支, 且该科在白垩纪-古近纪边界附近时期(约66.0 Ma)可能发生过快速辐射分化事件。

关键词: 苋科, 石竹目, 分化时间, 系统发育

Abstract:

Amaranthaceae sensu lato, including Amaranthaceae sensu stricto and Chenopodiaceae, is the second largest family in Caryophyllales. However, the family status of Chenopodiaceae is disputable and phylogenetic relationships among all of the subfamilies circumscribed within Amaranthaceae s.l. have not been well resolved to date. In the present study, phylogeny of Amaranthaceae s.l. was reconstructed based on a comprehensive taxonomic sampling of all 13 subfamilies (59 species) circumscribed using eight plastid DNA fragments, and a molecular dating analysis of the family was also conducted. Results revealed that, the monophyly of Amaranthaceae both in broad and narrow sense was strongly supported, but the monophyly of Chenopodiaceae was rejected. Thus the concept of Amaranthaceae s.l. is accepted here. Phylogenetic positions of all subfamilies were all resolved with strong support values, except that the position of the subfamily Polycnemoideae was weakly supported. Additionally, the crown age of Amaranthaceae s.l. was estimated at ca. 69.9 million years ago (Ma) in the late Cretaceous, and a period of rapid divergence may have occurred near the Cretaceous-Paleogene (K-Pg) boundary (ca. 66.0 Ma).

Key words: Amaranthaceae, Caryophyllales, molecular dating, phylogeny

黄久香,陈文娜,李玉玲,姚纲. 基于多个叶绿体基因序列片段重建广义苋科系统发育关系. 植物学报, 2020, 55(4): 457-467.

Jiuxiang Huang,Wenna Chen,Yuling Li,Gang Yao. Phylogenetic Study of Amaranthaceae sensu lato Based on Multiple Plastid DNA Fragments. Chinese Bulletin of Botany, 2020, 55(4): 457-467.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB19228

https://www.chinbullbotany.com/CN/Y2020/V55/I4/457

图/表 3

表1 本研究所用DNA序列相关信息

Table 1 Information of DNA sequences used in the study

Name of DNA sequences	Length of DNA sequences (bp)	Model selected
atpB	1497	GTR+I+Γ
ndhF	2242	TVM+I+Γ
psbB	1527	GTR+I+Γ
rbcL	1344	TVM+I+Γ
rpoC2	4147	GTR+I+Γ
rps4	606	TVM+Γ
rps16	1401	GTR+I+Γ
matK/trnK	2757	GTR+I+Γ

图1 基于8个叶绿体DNA序列片段构建的广义苋科系统发育树各分支节点上方数字分别代表ML树自展支持率/BI树后验概率。

Figure 1 Phylogeny of Amaranthaceae sensu lato inferred from the combined matrix of eight plastid DNA regions Bootstrap support value and posterior probability of each node are indicated above branches.

图2 基于8个叶绿体DNA序列片段及4个时间键准点采用BEAST分析所得广义苋科分化时间黑色圆点表示时间键准点所在位置。空心箭头示广义苋科冠群节点。Ach: 牛膝亚科; Aer: 白花苋亚科; Ama: 苋亚科; Bet: 甜菜亚科; Cam: 樟味藜亚科; Cel: 青葙亚科; Che: 藜亚科; Cor: 虫实亚科; Gom: 千日红亚科; Pol: 多节草亚科; Sali: 盐角草亚科; Sals: 猪毛菜亚科; Sua: 碱蓬亚科

Figure 2 Chronogram based on the BEAST analysis of the combined matrix of eight plastid DNA regions and four calibrations Calibration points are depicted with black circles. The crown node of Amaranthaceae sensu lato is shown by a hollow arrowhead. Ach: Achyranthoideae; Aer: Aervoideae; Ama: Amaranthoideae; Bet: Betoideae; Cam: Camphorosmoideae; Cel: Celosioideae; Che: Chenopodioideae; Cor: Corispermoideae; Gom: Gomphrenoideae; Pol: Polycnemoideae; Sali: Salicornioideae; Sals: Salsoloideae; Sua: Suaedoideae

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