植物铜转运蛋白的结构和功能

doi:10.11983/CBB15149

植物学报 ›› 2016, Vol. 51 ›› Issue (6): 849-858.DOI: 10.11983/CBB15149

植物铜转运蛋白的结构和功能

袁金红^1,², 李靖锐^2,³, 张海燕^1,^2,,A;^*()

¹天津师范大学生命科学学院, 天津 300387
²中国科学院植物研究所北方资源植物重点实验室, 北京 100093
³山西师范大学生命科学学院, 临汾 041000

收稿日期:2015-08-14 接受日期:2016-02-01 出版日期:2016-11-01 发布日期:2016-12-02
通讯作者: 张海燕
作者简介:
# 共同第一作者
基金资助:
国家自然科学基金(No.31470292, No.31270313)

Structure and Function of Copper Transporters in Plants

Jinhong Yuan^1,2, Jingrui Li^2,3, Haiyan Zhang^1,2*

¹College of Life Sciences, Tianjin Normal University, Tianjin 300387, China
²Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
³School of Life Sciences, Shanxi Normal University, Linfen 041000, China

Received:2015-08-14 Accepted:2016-02-01 Online:2016-11-01 Published:2016-12-02
Contact: Zhang Haiyan
About author:
# Co-first authors

摘要/Abstract

摘要： 铜(Cu)是植物必需的微量营养元素, 参与植物生长发育过程中的许多生理生化反应。Cu缺乏或过量都会影响植物的正常新陈代谢过程。因此, 植物需要一系列Cu转运蛋白协同作用以保持体内Cu离子的稳态平衡。通常, Cu转运蛋白可分为两类, 即吸收型Cu转运蛋白(如COPT、ZIP和YSL蛋白家族)和排出型Cu转运蛋白(如HMA蛋白家族), 主要负责Cu离子的跨膜转运及调节Cu离子的吸收和排出。然而, 最近有研究表明, 有些Cu伴侣蛋白家族可能是从Cu转运蛋白家族进化而来, 且它们在维持植物细胞Cu离子稳态平衡中也具重要功能。该文对Cu转运蛋白和Cu伴侣蛋白的表达、结构、定位及功能等研究进展进行综述。

Abstract: Copper (Cu) is an essential micronutrient involved in many physiological and biochemical reactions in growth and development in plants. Cu deficiency or excess can affect plant metabolism. Therefore, a series of Cu transporters are needed to act synergistically to sustain Cu homeostasis in plants. Plant Cu transporters can be classified into 2 general classes — Cu-uptake transporters (e.g., COPT, ZIP and YSL protein families) and Cu-efflux transporters (e.g., HMA protein family) — responsible for membrane transport of Cu and mediating Cu uptake and efflux. Recent studies have shown that some Cu chaperone protein families may be evolved from Cu transporter protein families, and Cu chaperones play an important role in maintaining Cu homeostasis. Here, we review developments in the expression, structure, location and function of Cu transporters as well as Cu chaperones.

袁金红, 李靖锐, 张海燕. 植物铜转运蛋白的结构和功能. 植物学报, 2016, 51(6): 849-858.

Jinhong Yuan, Jingrui Li, Haiyan Zhang. Structure and Function of Copper Transporters in Plants. Chinese Bulletin of Botany, 2016, 51(6): 849-858.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB15149

https://www.chinbullbotany.com/CN/Y2016/V51/I6/849

图/表 2

图1 COPT蛋白家族结构示意图(改自Peñarrubia et al., 2010)(A) 拟南芥COPT蛋白的序列比对; (B) COPT蛋白的拓扑结构; (C) 人类Ctr1同源三聚体TM2的空间结构。M: 蛋氨酸; G: 甘氨酸; C: 半胱氨酸

Figure 1 The COPT family of Cu transport proteins (modified from Peñarrubia et al., 2010)(A) Alignment of the putative Arabidopsis thaliana COPT Cu transporters; (B) The proposed topological structure of the COPT proteins; (C) The spatial disposition of TM2 in the human Ctr1 homotrimer complex. M: Met; G: Gly; C: Cys

图2 P1B-type ATPase的拓扑结构和主要结构域示意图(Arguello et al., 2007)H1-H8: 跨膜结构域; TM-MBS: 跨膜金属结合位点; N-MBD: N端金属结合域; C-MBD: C端金属结合域。A、P和N: P1B-type ATPase 的3个功能域

Figure 2 Schematic illustration of the topology and main domains present in P1B-type ATPases (Arguello et al., 2007)H1-H8: Transmembrane segments; TM-MBS: Transmembrane metal binding sites; N-MBD: N-terminal metal binding domain; C-MBD: C-terminal metal binding domain. A, P and N: The maindo-mains present in P1B-type ATPases

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植物铜转运蛋白的结构和功能

Structure and Function of Copper Transporters in Plants

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