植物学报 ›› 2015, Vol. 50 ›› Issue (6): 746-753.DOI: 10.11983/CBB15073

• 技术方法 • 上一篇    下一篇

一种快速定量分析药用植物淫羊藿生物碱的方法

孙晨倩1, 陈乐1, 张华峰1,,A;*(), 杨晓华2,,A;*(), 杨娟1   

  1. 1陕西师范大学食品工程与营养科学学院, 药用资源与天然药物化学教育部重点实验室/西北濒危药材资源开发国家工程实验室, 西安 710062
    2西安交通大学医学院, 卫生部法医学重点实验室, 西安 710061
  • 收稿日期:2015-04-22 接受日期:2015-08-10 出版日期:2015-11-01 发布日期:2015-09-06
  • 通讯作者: 张华峰,杨晓华
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    中央高校基本科研业务费专项资金(No;GK201503002)、陕西省科技合作计划(No.2014SJ-01, 2014SJ-08)、陕西省自然科学基础研究计划(No.2015JM3101)和国家级大学生创新创业训练计划(No.201510718017)

A Rapid Method for Quantitative Analysis of Alkaloids in Epimedium

Chenqian Sun1, Le Chen1, Huafeng Zhang1*, Xiaohua Yang2*, Juan Yang1   

  1. 1Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry/National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
    2Key Laboratory of Ministry of Health for Forensic Sciences, School of Medicine, Xi'an Jiaotong University, Xi’an 710061, China
  • Received:2015-04-22 Accepted:2015-08-10 Online:2015-11-01 Published:2015-09-06
  • Contact: Zhang Huafeng,Yang Xiaohua
  • About author:

    ? These authors contributed equally to this paper

摘要:

建立了基于近红外漫反射光谱的药用植物淫羊藿(Epimedium)生物碱的定量分析方法, 测定了9种淫羊藿生物碱的含量。当聚类分析采用主成分分析、数学处理采用2,4,4,1模式、光谱散射校正采用标准正则变换+散射处理及回归分析采用改进最小二乘法时, 淫羊藿生物碱近红外定标模型的定量效果最好; 定标方程的定标决定系数、交互验证相关系数、定标标准差和交互验证标准差分别为0.899 7、0.845 7、0.168 6和0.085 3。近红外定标模型预测值与常规化学分析值差异很小。应用近红外漫反射光谱法测得9种淫羊藿生物碱的含量介于6.537-20.586 mg·g-1 (干重)之间, 淫羊藿生物碱的种间差异较大。近红外漫反射光谱法具有快速、简便和无损(不损耗受检材料)的优点, 可用于淫羊藿生物碱的研究与开发。

Abstract:

A novel method for quantitative analysis of alkaloids in Epimedium was developed based on near-infrared reflectance spectroscopy. Content of alkaloids in nine species of Epimedium was quantified by the technique. Principal component analysis, ‘2,4,4,1’ mathematical treatment, standard normal variant and de-trending and modified partial least-squares analyses were used to establish the near-infrared model for quantifying alkaloids in Epimedium. The developed model of Epimedium alkaloids based on near-infrared spectroscopy possessed powerful predictive ability. The coefficient of determination for calibration, 1 minus variance ratio, standard error of calibration and standard error of cross-validation of calibration equation were 0.899 7, 0.845 7, 0.168 6 and 0.085 3, respectively. Alkaloid levels did not differ on analysis by the near-infrared reflectance spectroscopy method and conventional analytical method. Content of alkaloids in Epimedium samples ranged from 6.537 to 20.586 mg·g-1 dry weight. Alkaloid content varied greatly among the different Epimedium species. The proposed method based upon near-infrared spectroscopy is a fast, convenient and nondestructive alternative to the traditional analytical method and shows great potential in research and development of alkaloids in Epimedium.