Establishment of in vitro regeneration system of Helenium aromaticum
Chin Bull Bot. 2020, 55(3):
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Helenium aromaticum Hook Bailey has a fragrant odor, and the capitulum contains only disk flowers. It is a good material for studying the development of floral patterns about Compositae. However, there are few studies on its transgenic technology system. In order to establish an efficient in vitro regeneration system of the Helenium aromaticum. Induction of adventitious buds with different concentrations of 25 different phytohormone by using leaves, stem segments and hypocotyls as explants. The results showed that the leaves were explants, and the medium was MS+0.2 mg/L NAA+1 mg/L 6-BA+0.2 mg/L TDZ, The callus induction rate was as high as 100% after 20 days, and the induction rate of adventitious buds was 62.10%. The adventitious buds were placed in 1/2 MS medium for rooting culture, and the rooting rate reached 63.33% after 16 days. When cultivating until 30 days, the buds began to bloom and the flowering rate reached 93.33%. In addition, the study also showed that the regeneration of Helenium aromaticum was affected by explant, phytohormone type and concentration. 2,4-D is not conducive to the induction of adventitious buds, and the combination of suitable concentration of 6-BA and TDZ can effectively promote the formation of adventitious buds of Helenium aromaticum. This study preliminarily established an in vitro regeneration system under the condition of tissue culture of Helenium aromaticum, which laid a solid foundation for the subsequent establishment of its genetic transformation system, in order to be used for the study on the floral development patterns of Compositae.