Chin Bull Bot ›› 2009, Vol. 44 ›› Issue (06): 728-734.doi: 10.3969/j.issn.1674-3466.2009.06.011
• 技术方法 •
Yuanwei Fan1, Aizhi Liu2, Huafang Wang1
The uniquearbor tree species Populus euphratica can grow well in arid desert. We established an Agrobacterium based transformation system for this species using a GUS gene as the reporter. Shoots of P. euphratica were regenerated from leaves placed on 1/2 MS supplemented with 1.0 mmol.L-1 6-BA and 5.0 mmol.L-1 NAA, leaves were infected for 15 min in a solution of A. tumefaciens with 0.4-0.6 OD600 and 100 mmol.L-1 AS, and then co-cultured for 2 days. A. tumefaciens was inhibited with 300 mg.L-1 Cef, and transgenic tissues and shoots were selected with 9 mg.L-1 G418.
Yuanwei Fan;Aizhi Liu;Huafang Wang. Transformation of Populus euphratica[J].Chin Bull Bot, 2009, 44(06): 728-734.
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