Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (1): 19-29.doi: 10.3724/SP.J.1259.2014.00019
Guangjun Guo1†, Jianchang Gao1†, Xiaoxuan Wang1, Yanmei Guo1, John C. Snyder2, Yongchen Du1*
Solanum habrochaites, an important tomato germplasm resource, is resistant to multiple insect pests and produces an array of secondary metabolites on the leaf surface. We used gas chromatography-mass spectrometry (GC-MS) to determine the secondary metabolites on the leaf surface of 4 tomato accessions: one S. lycopersicum 9706 and 3 S. habrochaites accessions LA1777, LA2329 and PI134417. The 3 S. habrochaites accessions produced high amounts of secondary metabolites, whereas S. lycopersicum 9706 lacked most of these secondary metabolites. Secondary metabolites on the 9706 leaf surface were 6 terpenoids, which included 3 monoterpenes and 3 sesquiterpenes, of 60.3% and 39.7%, respectively. LA2329 and LA1777 accumulated high amounts of sesquiterpenoids, but the types of sesquiterpenes differed between accessions. α-zingiberene was the most abundant sesquiterpene (2 409.1 μg·g–1) in LA2329. γ-Elemene and E-β-farnesene were the major sesquiterpenes in LA1777: 573.3 and 289.9 μg·g–1, respectively. Dodecanoic acid ethenyl ester, at 5 312.8 μg·g–1, was the most abundant secondary metabolite in PI134417. This metabolite has not previously been reported in any tomato species. In addition, methyl ketones, specifically 2-undecanone and 2-tridecanone, at 689.8 and 1 459.7 μg·g–1, respectively, were abundant secondary metabolites in PI134417. Our study provides the theoretical basis for the use of tomato germplasm resources and the development of secondary metabolites.
Guangjun Guo, Jianchang Gao, Xiaoxuan Wang, Yanmei Guo, John C. Snyder, Yongchen Du. Secondary Metabolites on Leaf Surface of Different Tomato Species[J].Chin Bull Bot, 2014, 49(1): 19-29.
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