Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (2): 194-207.doi: 10.11983/CBB18184

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Effect of Iron Deficiency on the Protein Profile of Rice (Oryza sativa) Phloem Sap

Chen Lin,Lin Yan,Chen Pengfei,Wang Shaohua,Ding Yanfeng()   

  1. College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2018-08-28 Accepted:2019-01-11 Online:2019-09-01 Published:2019-03-01
  • Contact: Ding Yanfeng E-mail:dingyf@njau.edu.cn

Abstract:

In order to identify long-distance signals under iron (Fe) deficiency in rice (Oryza sativa), TMT label technique was used to study the protein profile of phloem sap under different iron concentrations. A total of 206 differentially ex- pressed proteins were identified: 54 were upregulated and 152 were downregulated. Most of these proteins are involved in hormone signal transduction, carbon metabolism, glutathione metabolism and mRNA transport. In addition, we mea- sured the physiological indicators according to the differentially expressed proteins. It was found that phytohormones, sucrose, glutathione and transporters were significantly changed under iron deficiency. To further study the function of these proteins can help to reveal the long distance signaling pathway of rice in response to Fe deficiency.

Key words: rice (Oryza sativa), iron deficiency, phloem, long-distance signal, TMT

Figure 1

Functional classification of the differentially expressed proteins of rice phloem sap via the Gene Ontology (GO) database "

Table 1

Differentially expressed proteins in phloem exudates of rice under iron deficiency in KEGG pathway"

Gene ID Protein name Unique peptides FC (-Fe/+Fe) P value
Plant hormone signal transduction
4332374 Probable protein phosphatase 2C 30 1 0.81 0.01
4333666 Regulatory protein NPR3 isoform X2 1 1.45 0.01
4337780 Probable indole-3-acetic acid-amido synthetase GH3.6 1 0.83 0.03
4340712 Gibberellin-regulated protein 5 1 1.22 0.00
Carbon metabolism
9268154 Malate synthase 12 0.77 0.03
4349723 Phosphoglycerate mutase-like protein 4 2 0.75 0.01
4345814 Alpha-amylase isozyme 3D 14 0.72 0.00
Stress and defense
4332474 L-ascorbate peroxidase 1, cytosolic 10 0.64 0.00
4336627 Probable phospholipid hydroperoxide glutathione peroxidase 2 0.75 0.00
4330235 Probable phospholipid hydroperoxide glutathione peroxidase 2 0.82 0.00
4349585 Cationic peroxidase 1 2 0.52 0.00
4347962 Peroxidase A2 isoform X2 8 0.80 0.00
4337232 Peroxidase 4 2 0.68 0.02
4332928 Peroxidase 2 1 0.58 0.00
4332175 Peroxidase A2 2 0.76 0.01
4344277 Peroxidase 2-like 5 0.82 0.00
4328425 4-hydroxyphenylpyruvate dioxygenase 5 0.82 0.02
Biosynthesis of amino acids
4342571 Tryptophan synthase alpha chain 1 0.79 0.05
Ribosome
4325555 50S ribosomal protein L13, chloroplastic 2 1.26 0.00
4324671 40S ribosomal protein S24-1 1 1.21 0.04
4334651 50S ribosomal protein L18, chloroplastic 2 1.21 0.04
3131445 Ribosomal protein S7 (plastid) 2 1.21 0.00
4342697 40S ribosomal protein S15a-1 1 1.28 0.02
3131440 Ribosomal protein S18 (plastid) 1 0.74 0.01
mRNA surveillance pathway
4347878 Polyadenylation and cleavage factor homolog 4 2 0.77 0.01
RNA transport
4329091 Eukaryotic translation initiation factor 1A 3 0.79 0.01
4345498 ABC transporter A family member 7 1 1.40 0.00

Figure 2

Schematic representation of plant hormone signal transduction in phloem of rice under iron deficiencyRed represents an increase of protein expression abundance, and yellow represents a decrease. The solid lines indicate direct action and the dotted lines indicate omission process. The arrows mean a positive effect and the short vertical lines mean inhibited effect."

Figure 3

The hormone content in rice shoots (A) and roots (B) after three days of iron deficiency treatmentDifferent lowercase letters indicate significant differences between iron deficiency (-Fe) and the control (+Fe)."

Figure 4

Metabolic pathways of carbon (A) and glutathione (B) related proteins in phloem exudates of rice under iron deficiencyYellow represents a decrease of protein expression abundance. The solid lines indicate direct action and the dotted lines indicate omission process. The arrows mean a positive effect."

Figure 5

The relative expression levels of OsIRT1, OsYSL15, OsNAS1 and OsNAS2 in root (A) and the relative expression levels of OsYSL2 and OsNAS1 in shoot (B) after three days of iron deficiency treatmentThe column diagram represents the relative gene expression level ratio of iron deficiency induced to the control."

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