新疆阿魏特征显微结构的三维原位无损研究

doi:10.11983/CBB17104

植物学报 ›› 2018, Vol. 53 ›› Issue (3): 364-371.DOI: 10.11983/CBB17104

所属专题：药用植物专辑 (2018年53卷3期)

新疆阿魏特征显微结构的三维原位无损研究

刘慧强^1,^*(), 凯撒·苏来曼², 孙芸³, 庞渊⁴, 樊孝喜¹, 谢茹¹, 柳超¹, 段颖妮¹, 马燕¹

¹新疆医科大学医学工程技术学院, 乌鲁木齐 830011
²新疆维吾尔自治区中药民族药研究所, 乌鲁木齐 830002
³新疆医科大学中医学院, 乌鲁木齐 830011
⁴乌鲁木齐市中医医院, 乌鲁木齐 830000

收稿日期:2017-05-23 接受日期:2017-10-07 出版日期:2018-05-01 发布日期:2018-09-11
通讯作者: 刘慧强

3-D In-situ Non-destructive Structural Characterization of Ferula sinkiangensis

Liu Huiqiang^1,^*(), Sulaiman·Kaisa², Sun Yun³, Pang Yuan⁴, Fan Xiaoxi¹, Xie Ru¹, Liu Chao¹, Duan Yingni¹, Ma Yan¹

¹College of Medical Engineering and Technology, Xinjiang Medical University, Urumqi 830011, China
²Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine and Ethno-Medicine, Urumqi 830002, China
³College of Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China
⁴Urumqi Hospital of Traditional Chinese Medicine, Urumqi 830000, China

Received:2017-05-23 Accepted:2017-10-07 Online:2018-05-01 Published:2018-09-11
Contact: Liu Huiqiang

摘要/Abstract

摘要： 利用新型第三代高亮度同步辐射光源相衬成像技术, 可实现弱吸收(主要含碳、氢、氧和氮等元素)物质材料特征结构的无损三维鉴定。以具有极高药用价值的新疆地产药材——阿魏(Ferula)为研究对象, 采用同步辐射高分辨X射线相衬显微CT技术, 并结合相位恢复算法, 有效解析和评价了新疆阿魏(F. sinkiangensis)的三维特征结构, 极大提高了对药材类生物样品的显微结构和密度的分辨能力, 从而为新疆阿魏的特征分辨、品质鉴定和真伪识别提供了一种直观可靠的三维可视化新手段。

关键词: 同步辐射光源, 相衬显微CT, 相位恢复, 显微结构鉴定, 新疆阿魏

Abstract: Synchrotron-based X-ray phase-contrast micro-tomography is being used for achieving nondestructive and 3-D characterization due to the high contrast imaging of low Z materials (consisting of C, H, O, N elements). In this paper, we present a new method to combine the high resolution synchrotron-based X-ray phase-contrast imaging technology and phase retrieval algorithm for analyzing and evaluating the 3D inner micro-structures and nondestructive characteristic structures of Ferula sinkiangensis. The method successfully revealed the 3-D micro-structures and characteristics of F. sinkiangensis with high-density resolution, demonstrating that the method is an intuitive and reliable tool of 3D visualization, which has good potential for characterizing and identifying Chinese medicine materials.

Key words: synchrotron radiation source, phase-contrast micro-tomography, phase retrieval, microscopic identification, Ferula sinkiangensis

刘慧强, 凯撒·苏来曼, 孙芸, 庞渊, 樊孝喜, 谢茹, 柳超, 段颖妮, 马燕. 新疆阿魏特征显微结构的三维原位无损研究. 植物学报, 2018, 53(3): 364-371.

Liu Huiqiang, Sulaiman·Kaisa, Sun Yun, Pang Yuan, Fan Xiaoxi, Xie Ru, Liu Chao, Duan Yingni, Ma Yan. 3-D In-situ Non-destructive Structural Characterization of Ferula sinkiangensis. Chinese Bulletin of Botany, 2018, 53(3): 364-371.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB17104

https://www.chinbullbotany.com/CN/Y2018/V53/I3/364

图/表 8

图1 新疆阿魏药用部位(A)与实验样品(B)

Figure 1 The medicinal part of Ferula sinkiangensis (A) and experimental samples (B)

图2 上海光源X射线成像及生物医学应用线站(BL13W1)实验光路示意图(A)和基于标量衍射的同轴相衬成像(PPCT)原理图(B)

Figure 2 Schematic of X-ray imaging and biomedicine application Beamline (BL13W1) at Shanghai Synchrotron Radiation Facility (SSRF) (A) and the principle of propagation-based phase-contrast computed tomography (PPCT) (B)

图3 上海光源X射线成像与生物医学应用线站(BL13W1)实验平台包括高精度六维样品台(样品置于红色椭圆处的套管中)以及高分辨X射线探测器。

Figure 3 Experimental platform of biomedicine application Beamline (BL13W1) at Shanghai Synchrotron Radiation FacilityThe platform included high-precision sample stage (samples located in the tube marked with red circle) and high-resolution X-ray detector.

图4 新疆阿魏根茎部同轴相衬显微CT成像(PPCT)重构结果的三切面图(A) 横向切割图; (B) 冠状面切割图; (C) 子午面切割图。1: 导管; 2: 内涵韧皮部薄壁细胞; 3: 木栓纤维束; 4: 淀粉粒; 5: 微阿魏酸酯簇晶(此图仅是样品重构切片的某一层, 具有代表性, 可观察到密度较高的阿魏酸酯簇晶, 表现为高亮度的像素分布)。Bars=150 µm

Figure 4 Slices of Propagation-based Phase-contrast Com- puted Tomography (PPCT) reconstructed results of root-stem position of Ferula sinkiangensis(A) Transverse section; (B) Coronary section; (C) Saggital section. 1: Vessels; 2: Included phloem parenchyma cells; 3: Xylem fiber bundles; 4: Starch grain; 5: Clusters of oryza- nolum crystal (a representative image showing the distribution of high density oryzanolum crystal with bright pixels). Bars=150 μm

图5 新疆阿魏根茎部同轴相衬显微CT成像(PPCT)三维渲染重构体视图(A) 根茎部各组分的彩色显微特征体结构(其中浅蓝色代表植物细胞壁和管径, 深蓝色代表植物导管空腔和孔隙); (B), (C) 基于相位恢复算法定量提取的纤维束导管(蓝色)、淀粉粒(蓝紫色)及阿魏酸酯簇晶(红色)不同角度的三维结构及特征分布。

Figure 5 3D-renderings of Propagation-based Phase-contrast Computed Tomography (PPCT) reconstructed results of root-stem position of Ferula sinkiangensis(A) Volumetric visualization of the whole sample with different colors (light blue denotes plant cell walls and ducts; dark blue denotes duct cavities and holes); (B), (C) Quantitative segmentation of xylem fiber bundles (blue color), starch grain (bluish violet color) and oryzanolum crystal (red color) with different views based on phase retrieval algorithm.

图6 新疆阿魏茎节叉部同轴相衬显微CT成像(PPCT)重构结果的三切面图(A) 横向切割图; (B) 冠状面切割图; (C) 子午面切割图。白色实线箭头示节结分叉线, 分布高密度阿魏酸酯晶粒(白色高亮的颗粒分布); 白色虚线箭头示横向纤维束, 呈射线状, 内含纵向微管束; 白色长点箭头示高致密管状木栓纤维束, 内含圆形薄壁细胞群。Bars=150 µm

Figure 6 Slices of Propagation-based Phase-contrast Com- puted Tomography (PPCT) reconstructed results of stem bifurcation position of Ferula sinkiangensis(A) Transverse section; (B) Coronary section; (C) Saggital section. White solid arrows denote branch lines with high gray level, composed of oryzanolum crystal; White dash arrows denote transversal fiber bundles, including longitudinal micro-tubes; White long dot arrows denote high dense xylem fiber bundles with circular parenchyma cell group. Bars=150 μm

图7 新疆阿魏茎节叉部同轴相衬显微CT成像(PPCT)三维渲染重构体视图(A) 不同颜色代表茎节叉部各组分的显微特征体结构(浅蓝色代表植物细胞壁和管径, 深蓝色代表植物导管空腔和孔隙); (B), (C) 基于相位恢复算法的定量提取分叉线(蓝紫色)及横向纤维束(红色)的不同角度三维结构及特征分布。

Figure 7 3D-renderings of Propagation-based Phase-con- trast Computed Tomography (PPCT) reconstructed results of stem bifurcation position of Ferula sinkiangensis(A) Volumetric visualization of the whole sample with different colors (light blue denotes plant cell walls and ducts, dark blue denotes duct cavities and holes); (B), (C) Quantitative segmentation of bifurcation lines (bluish violet color) and transversal xylem fiber bundles (red color) with different views based on phase retrieval algorithm.

图8 基于相位恢复的新疆阿魏茎种子部同轴相衬显微CT成像(PPCT)三维渲染重构体视图(不同颜色代表种子各组分的显微结构特征: 由外及里为种皮、子叶、胚芽、淀粉粒和油管等结构)(A) 种子顶部横断面图; (B) 种子中部横断面图; (C) 种子内淀粉粒体视图和子叶组织; (D) 种子内油管的三维结构及特征分布

Figure 8 3D-renderings of Propagation-based Phase-con- trast Computed Tomography (PPCT) reconstructed results of seeds of Ferula sinkiangensis (different colors showing the seed coat, cotyledon, embryo, starch grain and pipeline from outside to inside of seed) (A) Volumetric visualization of the top part of seed sample; (B) The central part of seed sample; (C) 3D visualization of grain starch and cotyledon in seed; (D) 3D visualization and characteristic distribution of oil tubes in seed.

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新疆阿魏特征显微结构的三维原位无损研究

3-D In-situ Non-destructive Structural Characterization of Ferula sinkiangensis

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