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Chinese Bulletin of Botany >

2025 , Vol. 60 >Issue 1: 49 - 61

DOI: https://doi.org/10.11983/CBB24019

EXPERIMENTAL COMMUNICATIONS

Analysis of Expression Characteristics and Identification of Interaction Proteins of BnaABF2 Transcription Factor in Brassica napus

  • Liuqing Yang ,
  • Jin Wang ,
  • Jingli Yan ,
  • Qinqin Chen ,
  • Haokun Cheng ,
  • Chun Li ,
  • Peiyu Zhao ,
  • Bo Yang ,
  • Yuanqing Jiang
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  • College of Life Sciences, Northwest A & F University, Yangling 712100, China
†These authors contributed equally to this paper

Received date: 2024-02-05

  Accepted date: 2024-08-20

  Online published: 2024-08-22

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Abstract

ABF transcription factors are collectively referred to as basic leucine zipper proteins that can specifically recognize and bind to ABA-responsive elements (ABRE), participating in ABA signal transduction and serving as regulators of ABA signal transcriptional responses. This study analyzed the protein encoded by the BnaABF2 gene in Brassica napus. Subcellular localization results showed that the BnaABF2 protein is localized in the nucleus. Analysis of transcriptional activity in the yeast system indicated that BnaABF2 has no transcriptional activation activity; qRT-PCR detection revealed that the expression level of BnaABF2 is highest in leaves. We also found that ABA treatment, simulated drought, and salt stress can induce the expression of BnaABF2; BiFC results showed that BnaMPK1/2/6/7/9/12/13 can interact with BnaABF2. Dual-LUC results suggested that BnaMPK7 may enhance the transcriptional regulation of BnaABF2 on downstream target genes through phosphorylation. This study initially explored the basic characteristics and interacting proteins of the transcription factor BnaABF2, providing theoretical guidance for understanding its functions and mechanisms.

Key words： Brassica napus; ABF2; subcellular localization; MPK; interaction proteins

Cite this article

Liuqing Yang , Jin Wang , Jingli Yan , Qinqin Chen , Haokun Cheng , Chun Li , Peiyu Zhao , Bo Yang , Yuanqing Jiang . Analysis of Expression Characteristics and Identification of Interaction Proteins of BnaABF2 Transcription Factor in Brassica napus[J]. Chinese Bulletin of Botany, 2025 , 60(1) : 49 -61 . DOI: 10.11983/CBB24019

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