Morphological Identification and Development of Linkage Markers for Lobed Ray Floret Mutants in Marigold (Tagetes erecta)
Received date: 2022-10-03
Accepted date: 2023-03-07
Online published: 2023-03-10
Marigold (Tagetes erecta) is an important ornamental plant of the genus Tagetes in the Asteraceae. The morphology of its ray floret corolla varies from smooth, undulate to different degree of incision. An F2 population was constructed from a cross between the mutant marigold JH with five-lobed corollas and fertile stamens in the first round florets and the inbred line S5 with smooth corolla of ray florets and aborting stamens. Inheritance analysis showed that the mutation trait of JH was controlled by a single-dominant gene, named Tagetes erecta lobed ray florets (Telf). Histological and cytological observations showed that the first round ray florets of JH were disk-like, with five lobes formed at the top of the corolla tube, the middle and lower parts were tubular, and the development of anthers and pollen grains are to normal. Using BSR-seq and comparative genomics methods, the gene Telf controlling lobed ray florets was mapped in the interval of 37003-SCAR and 34032-CAPS. 37003-SCAR marker was 3.684 cM from the Telf gene. On the other side of the gene, 34032-CAPS marker was placed about 3.517 cM from Telf. This study laid a foundation for the subsequent fine mapping of the target gene Telf, and also provided a new method for the selection of molecular markers in marigold.
Key words： Tagetes erecta; ray floret; lobed corolla; BSR-seq; molecular marker
Wenjing Wang, Yu Zhu, Hongming Zhang, Ludan Wei, Qingping Yi, Xiaomin Yu, Yuhan Liu, Lixue Zhang, Wenhan Cheng, Yanhong He . Morphological Identification and Development of Linkage Markers for Lobed Ray Floret Mutants in Marigold (Tagetes erecta)[J]. Chinese Bulletin of Botany, 2023 , 58(6) : 893 -904 . DOI: 10.11983/CBB22231
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