Changes of Small GTPases Activity During Cucumber Tendril Winding
Received date: 2022-03-27
Accepted date: 2022-05-10
Online published: 2022-05-10
Tendrils of climbing plants are highly sensitive to mechanical stimuli. Small GTPases signals can be activated by mechanical stimulation which regulate development in Arabidopsis, but whether small GTPases signals play a role in the entanglement of tendrils response to mechanical stimuli is unknown. In this study, the activity of small GTPases in cucumber tendril after mechanical stimulation was investigated using Cucumis sativus, and ROP activity detection results showed that the activity of small GTPases CsROP6 was significantly enhanced during tendril winding. Meanwhile, mass spectrometry revealed that auxin content increased significantly during tendril winding. Further experiments showed that exogenous auxin treatment increased the activity of CsROP6, suggesting mechanical stimulation might activate ROP6 activity through auxin during tendril winding. This study suggests that mechanical stimulation of small GTPases signals may be conserved in different plants.
Key words： auxin; cucumber tendrils; mechanical signal; small GTPases
Binqi Li, Jiahui Yan, Hao Li, Wei Xin, Yunhe Tian, Zhenbiao Yang, Wenxin Tang . Changes of Small GTPases Activity During Cucumber Tendril Winding[J]. Chinese Bulletin of Botany, 2022 , 57(3) : 299 -307 . DOI: 10.11983/CBB22058
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