Chinese Bulletin of Botany >

2022 , Vol. 57 >Issue 2: 227 - 235

DOI: https://doi.org/10.11983/CBB21069

TECHNIQUES AND METHODS

Optimization of Cell Suspension Culture Conditions of Vaccinium dunalianum

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  • 1Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China
    2Key Laboratory of State Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China
    3Collaborative Innovation Center of Forest Resources Breeding and Utilization in Yunnan, Kunming 650224, China

Received date: 2021-04-27

  Accepted date: 2021-11-24

  Online published: 2021-11-24

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Abstract

To increase the biomass of suspension culture of Vaccinium dunalianum, we used callus induced from leaves as start material, and investigated the effects of sucrose concentration, pH value, culture medium volume, initial inoculation size, and shaking speed using single factor experiment method. We optimized the suspension culture conditions using Box-Behnken response surface methodology. Our results showed that the optimal conditions for cell suspension culture were: 40 g·L-1 sucrose, pH5.2, culture medium volume 45 mL, callus inoculation size of 2.64 g, and shaking speed of 149 r·min-1 based on the improved WPM medium. Under these optimal conditions, the dried cell biomass was 0.184 4 g which was close to the theoretical prediction of 0.184 5 g, and the growth curve of cells showed ‘S’ type. These results provide a basis for further study on the regulation of the secondary metabolites of cell suspension culture in V. dunalianum.

Key words： Vaccinium dunalianum; callus; cell suspension culture; culture condition; response surface methodology

Cite this article

Churan Li, Ling Fu, Yun Liu, Xiaoqin Yang, Guolei Zhu, Sida Xie, Huancheng Ma, Ping Zhao . Optimization of Cell Suspension Culture Conditions of Vaccinium dunalianum[J]. Chinese Bulletin of Botany, 2022 , 57(2) : 227 -235 . DOI: 10.11983/CBB21069

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