Identification and Mapping of a Rice Male Sterility Mutant ms102
Received date: 2021-09-14
Accepted date: 2021-11-17
Online published: 2021-11-17
The recessive nuclear male sterility line is the base of the third-generation hybrid rice breeding technology. To explore new male sterility lines suitable for the breeding technology, we screened an ethyl methylsulfonate (EMS) mutant library derived from the elite xian variety Huanghuazhan (HHZ) and identified male sterility mutant 102 (ms102). The ms102 showed a normal vegetative development, but exhibited defective anther dehiscence and pollen abortion. Cytological analyses revealed that the degradation of the tapetum was abnormal in ms102, resulting in the abortion of microspores. Genetic analyses showed that the male sterile phenotype of ms102 was resulted from the mutation of a known male sterility gene Defective Pollen Wall 2 (DPW2), which encodes an acyl transferase. Thus, this study identified a recessive nuclear male sterility mutant, and further confirmed the function of DPW2 in rice pollen development.
Key words： rice; male sterility; gene cloning
Xia Wang, Wei Yan, Zhiqin Zhou, Zhenyi Chang, Minting Zheng, Xiaoyan Tang, Jianxin Wu . Identification and Mapping of a Rice Male Sterility Mutant ms102[J]. Chinese Bulletin of Botany, 2022 , 57(1) : 42 -55 . DOI: 10.11983/CBB21158
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