Expression of Amaranthin-like Lectins Gene and Responses to Abiotic Stresses in Cucumber
Received date: 2020-11-01
Accepted date: 2021-03-01
Online published: 2021-03-01
Lectins are globular proteins with carbohydrate-binding sites which enable them to specifically recognize and bind single or more particular carbohydrate structures. Previous studies have shown that lectins play critical roles in plant defense against their herbivores and pathogens. Due to their toxicity towards organisms like bacteria, fungi, viruses and insects, lectins are believed to have great potential in both agricultural and medical applications. As one of the smallest lectin families, there has been very limited research on amaranthin-like lectins. Here, we analyzed the distributions and intron/exon structures from 16 different amaranthin-like genes in the genome of cucumber, an important economic crop. To evaluate their responses against different stresses/plant hormones, promoter analysis was performed for all amaranthin-like genes. Finally, real-time quantitative PCR were performed on stress-treated plants to analyze the responses of amaranthin-like genes towards cold, salt, drought stresses and ABA treatment. This work provides valuable information for the study of physiological roles of amaranthin-like proteins and their involvement in plant defense.
Key words： abiotic stress; amaranthin-like lectins; promoter; protein domain
Fei Zhao, Liuyi Dang, Minhui Wei, Chunying Liu, Wei Leng, Chenjing Shang . Expression of Amaranthin-like Lectins Gene and Responses to Abiotic Stresses in Cucumber[J]. Chinese Bulletin of Botany, 2021 , 56(2) : 183 -190 . DOI: 10.11983/CBB20177
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