To develop and optimize the technology to extract lappaconitine and ranaconitine from Aconitum sinomontanum Nakai, we detected comprehensive extraction rates as an index by high-performance liquid chromatography. The orthogonal test was used for optimizing the extraction technology. Lappaconitine and ranaconitine were extracted with 10 times 80% methanol and 2% material amount of HCl added to the material powder, then refluxed for 6 h under 75°C. The optimized technique is stable and can be used for extracting active components from A. sinomontanum.
Bin Xie, Junhui Zhou, Xinwei Shi, Sifeng Li
. Optimization of Extracting Technology for the Active Components in Aconitum sinomontanum[J]. Chinese Bulletin of Botany, 2014
, 49(2)
: 198
-202
.
DOI: 10.3724/SP.J.1259.2014.00198
[1]周军辉,李静,王新莉,王答祺,李思锋 .HPLC法测定高乌头药材中高乌甲素[J].中草药,2007,38(9):1414-1415
[2]唐希灿,朱梅英,冯洁.刺乌头碱氢澳酸盐的药理作用研究[J].药学学报,1983,18:579-581
[3]刘建华,朱悦心,唐希灿.N-脱乙酰刺乌头碱和刺乌头碱的抗炎和镇痛作用. [J].中国药理学报,1987,8:301-305
[4]Ono M., Satoh T..Pharmacological studies of lappaconitine Analgesic activities[J].Arzneim–Forsch / Drug Res,1988,38:892-895
[5]张慧灵,王翔毅,杨玉梅,刘和莉,徐继辉.冉乌头碱的镇痛作用及其对麻醉大鼠心电图的影响[J].包头医学院学报,2001,16(4):261-264
[6]Yang Li,Jun-hui Zhou,Gui-jun Han,Min-juan Wang,Wen-ji Suna and Ye Zhao.X-ray Crystallographic Study of Ranaconitine[J].Natural Product Communications,2011,6 (11):1589-1590
[7]韦璧瑜,孔宪武,赵志远,王洪诚,朱任宏 (1980).高乌头生物碱的研究一[J].西北师范大学学报(自然科学版),1980,01:80-88
Home