Genome-wide Identification and Analysis of CONSTANS-like Gene Family in Nicotiana tabacum
Received date: 2020-08-25
Accepted date: 2021-01-05
Online published: 2021-01-05
Nicotiana tabacum (tobacco) is one of the model plants for molecular biology research as well as an important economic crop in the world. A suitable living environment is essential for the growth and reproduction of tobacco. CONSTANS-like (COL) family proteins are not only key regulators for flowering time, but also play important roles in stress response of plants. Our aim was to identify the COL family members, analyze their gene structure, evolutionary relationship, transcriptional regulatory elements and expression patterns, and explore their possible functions in response to cold stress in tobacco. We identified a total of 15 COL genes with similar physiochemical properties in tobacco. Evolutionary analysis divided all COL genes into three categories, and similar intron structure and motif distribution were observed among genes within each category. The promoter regions of tobacco COL genes contain a large number of cis-acting elements related to responses to light, low temperature, drought and phyto hormone. Gene expression analysis showed that low temperature significantly affected the expression of COL genes in tobacco, but the effects on different genes were different. Our study showed different parental (N. sylvestris (maternal) and N. tomentosiformis (paternal)) expression bias between different COL genes in tobacco, and most of the bias patterns were maintained from 6-7 leaf stage to budding stage.
Key words： Nicotiana tabacum; COL; phylogeny; low temperature; expression bias
Yawen Zhang, Shan Liang, Guoyun Xu, Wuxia Guo, Shulin Deng . Genome-wide Identification and Analysis of CONSTANS-like Gene Family in Nicotiana tabacum[J]. Chinese Bulletin of Botany, 2021 , 56(1) : 33 -43 . DOI: 10.11983/CBB20147
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