A regeneration system for organogenesis and somatic embryogenesis in vitro was established by using leaves of Agapanthus praecox as explants, and different cultivation media for transplanting were selected for the best effect. The results showed that picloram (PIC) was effective in callus induction of leaves, and the optimal medium was MS+2.0 mg·L ^-1 PIC. The callus induction rate was determined by the meristematic activity of leaf segments. The callus induction rate of the basal tissues on the 1 ^st-2 ^nd euphyll was 85.71%, and the callus induction rate was 66.48% in meristematic zone of 0-0.5 cm of the same leaf. The results also showed that the callus induction efficiency was higher in the middle of leaf transection compared with that at the edge. The optimal medium for adventitious bud induction was MS+1.5 mg·L ^-1 PIC+0.3 mg·L ^-1 6-BA, and the induction rate was 80.27%. The basic MS medium was suitable for somatic embryo induction, but the induction rate would be significantly increased if 0.05 mg·L ^-1 paclobutrazol and 1.0 mg·L ^-1abscisic acid were added. Plantlets proliferation was promoted by 1.0 mg·L ^-1 6-BA, and the proliferation coefficients of organogenesis and somatic embryogenesis pathway were 2.23 and 2.93, respectively. The combination of peat:perlite: vermiculite=1:1:1 (v/v/v) was proved the suitable substrate for transplanting and acclimatization of plantlets, with a survival rate of 100%. This regeneration system provides a rapid and efficient propagation technology for A. praecox, and also provides a reference for the regeneration of monocotyledon explants in vitro.