The Genetic Diversity of Common Millet (Panicum miliaceum) Germplasm Resources Based on the EST-SSR Markers
Received date: 2019-02-24
Accepted date: 2019-06-18
Online published: 2019-06-18
The EST-SSR molecular markers of common millet (Panicum miliaceum) were developed by high-throughput sequencing. Using these markers, we assessed the genetic diversity in a panel of 144 common millet accessions collected from different ecotopic regions in China and abroad. It was shown that 80 pairs of these markers were polymorphic, with the efficiency of approximately 40%. The resolution power (Rp) was 0.67-4.67 (mean 2.00) and the amplified product sizes ranged from 50 to 500 bp. Among the examined 144 accessions, 206 allelic variations were identified in 80 loci, with 2-3 alleles at each locus. The Shannon’s diversity index (I) ranged from 0.659 3 (RYW108) to 1.087 2 (RYW124) with an average of 0.859 9. The range of polymorphism information content (PIC) was 0.222 9 (RYW98) -0.717 2 (RYW124) with an average of 0.457 3. Based on UPGMA, these 144 accessions were classified into 3 groups, two of which belonged to the the Northern China spring-sowing ecotopes and one group was mainly from the Loess Plateau spring-summer-sowing ecotopes. Based on Structure (K=4), all the accessions were divided into four groups, of which two groups represented the gene pool originated from the Northern China, whereas the other two groups from the Loess Plateau and abroad accessions. Based on principal component analysis (PCA), the accessions were clustered into seven groups, consistent with their geographic origins.
Key words： Panicum miliaceum; genetic diversity; PCA; EST-SSR markers
Jieli He,Tiantian Shi,Ling Chen,Haigang Wang,Zhijun Gao,Meihong Yang,Ruiyun Wang,Zhijun Qiao . The Genetic Diversity of Common Millet (Panicum miliaceum) Germplasm Resources Based on the EST-SSR Markers[J]. Chinese Bulletin of Botany, 2019 , 54(6) : 723 -732 . DOI: 10.11983/CBB19037
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