Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum
Received date: 2018-11-29
Accepted date: 2019-03-05
Online published: 2019-03-26
We investigated the effects of different explants, kinds and concentrations of plant growth regulators on callus induction and plant regeneration from scales of Lilium bakerianum var. aureum at tissue culture. The medium MS+1.0 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1 sucrose was suitable for inducing adventitious buds and calli. The medium MS+0.5 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1 sucrose was suitable for adventitious bud multiplication. MS+1.0 mg∙L -1NAA+30 g∙L -1 sucrose was optimal for bulblet formation from adventitious buds, and the optimal medium for the growth of bulblets was 1/2MS+0.01 mg∙L -1NAA+60 g∙L -1 sucrose. We provide technical support for germplasm resource protection and rapid propagation of L. bakerianum var. aureum.
Wenting Zhang,Yanhong He,Ning Shu,Jingjing Xing,Baojun Liu,Manzhu Bao,Guofeng Liu . Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum[J]. Chinese Bulletin of Botany, 2019 , 54(6) : 773 -778 . DOI: 10.11983/CBB18256
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