Shoot segments with axillary buds from young branches of wild Ardisia violacea with excellent characters were cultured in vitro. Through bud induction, multiple shoots proliferation, subculture, rooting and transplantation, a regeneration system was established. The optimal bud induction medium was MS+0.80 mg?L^-1 KT+0.10 mg?L^-1 NAA+0.10 mg?L^-1 IBA, and the germination frequency of axillary buds reached 92.60%; the optimal multiple shoot proliferation medium was MS1+0.50 mg?L^-1 TDZ+0.10 mg?L^-1 NAA, and the average proliferation coefficient reached 8.60; the optimal subculture medium was MS+1.00 mg?L^-1 KT+0.50 mg?L^-1 NAA; the optimal rooting medium was 1/2MS+2.00 mg?L^-1 IBA+1.00 mg?L^-1 NAA+1.00 mg?L^-1 AC, and the average frequency of shoot rooting reached 98.70%. Using pine bark:peat (2:1, v/v) as a matrix, the transplant survival rate could reach 85.30%. We established a high-frequency regeneration system for A. violacea that could meet the needs of large-scale production.