H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana

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  • Key Laboratory of Plant Biotechnology in Universities of Shandong, College of Life Science, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2011-09-01

  Revised date: 2011-12-20

  Online published: 2012-07-06

Supported by

the National Science Foundation

Abstract

Using the Arabidopsis thaliana wild-type; mutants atrbohD, atrbohF, atrbohD/F, atl-cdes, and atd-cdes; and D-/L-cysteine desulfhydrase overexpression plants, we investigated the roles of hydrogen sulfide (H2S) in drought-induced stomatal closure in A. thaliana. We used a pharmacological experiment combined with laser scanning confocal microscopy and spectrophotography. The H2S scavenger hypotaurine and H2S synthesis inhibitors aminooxy acetic acid, hydroxylamine, potasium pyruvate and ammonia all inhibited drought-induced stomatal closure. Drought-induced stomatal closure was greater in overexpression than wild-type plants, with the effect not obvious in atl-cdes and atd-cdes. Moreover, drought stress enhanced the level of hydrogen peroxide (H2O2) in guard cells and increased the content of H2S and the activity and expression of D-/L-cysteine desulfhydrase in leaves but had no significant effect in atrbohD, atrbohF, and atrbohD/F. The H2O2 scavenger decreased drought-induced H2S production and the activity of D-/L-cysteine desulfhydrase in leaves. H2S may function downstream of H2O2 in the signal transduction pathway of drought-induced stomatal closure in A. thaliana.

Cite this article

Lanxiang Wang, Zhihui Hou, Lixia Hou, Fanggui Zhao, Xin Liu . H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana[J]. Chinese Bulletin of Botany, 2012 , 47(3) : 217 -225 . DOI: 10.3724/SP.J.1259.2012.00217

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