Chinese Bulletin of Botany ›› 2012, Vol. 47 ›› Issue (3): 217-225.DOI: 10.3724/SP.J.1259.2012.00217

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H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana

Lanxiang Wang, Zhihui Hou, Lixia Hou, Fanggui Zhao, Xin Liu*   

  1. Key Laboratory of Plant Biotechnology in Universities of Shandong, College of Life Science, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2011-09-01 Revised:2011-12-20 Online:2012-05-01 Published:2012-07-06
  • Contact: Xin Liu
  • Supported by:

    the National Science Foundation

Abstract: Using the Arabidopsis thaliana wild-type; mutants atrbohD, atrbohF, atrbohD/F, atl-cdes, and atd-cdes; and D-/L-cysteine desulfhydrase overexpression plants, we investigated the roles of hydrogen sulfide (H2S) in drought-induced stomatal closure in A. thaliana. We used a pharmacological experiment combined with laser scanning confocal microscopy and spectrophotography. The H2S scavenger hypotaurine and H2S synthesis inhibitors aminooxy acetic acid, hydroxylamine, potasium pyruvate and ammonia all inhibited drought-induced stomatal closure. Drought-induced stomatal closure was greater in overexpression than wild-type plants, with the effect not obvious in atl-cdes and atd-cdes. Moreover, drought stress enhanced the level of hydrogen peroxide (H2O2) in guard cells and increased the content of H2S and the activity and expression of D-/L-cysteine desulfhydrase in leaves but had no significant effect in atrbohD, atrbohF, and atrbohD/F. The H2O2 scavenger decreased drought-induced H2S production and the activity of D-/L-cysteine desulfhydrase in leaves. H2S may function downstream of H2O2 in the signal transduction pathway of drought-induced stomatal closure in A. thaliana.

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