H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana

doi:10.3724/SP.J.1259.2012.00217

Abstract

Abstract: Using the Arabidopsis thaliana wild-type; mutants atrbohD, atrbohF, atrbohD/F, atl-cdes, and atd-cdes; and D-/L-cysteine desulfhydrase overexpression plants, we investigated the roles of hydrogen sulfide (H₂S) in drought-induced stomatal closure in A. thaliana. We used a pharmacological experiment combined with laser scanning confocal microscopy and spectrophotography. The H₂S scavenger hypotaurine and H₂S synthesis inhibitors aminooxy acetic acid, hydroxylamine, potasium pyruvate and ammonia all inhibited drought-induced stomatal closure. Drought-induced stomatal closure was greater in overexpression than wild-type plants, with the effect not obvious in atl-cdes and atd-cdes. Moreover, drought stress enhanced the level of hydrogen peroxide (H₂O₂) in guard cells and increased the content of H₂S and the activity and expression of D-/L-cysteine desulfhydrase in leaves but had no significant effect in atrbohD, atrbohF, and atrbohD/F. The H₂O₂ scavenger decreased drought-induced H₂S production and the activity of D-/L-cysteine desulfhydrase in leaves. H₂S may function downstream of H₂O₂ in the signal transduction pathway of drought-induced stomatal closure in A. thaliana.

CLC Number:

Q945

Lanxiang Wang, Zhihui Hou, Lixia Hou, Fanggui Zhao, Xin Liu. H₂S Induced by H₂O₂ Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana[J]. Chinese Bulletin of Botany, 2012, 47(3): 217-225.

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https://www.chinbullbotany.com/EN/Y2012/V47/I3/217

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H₂S Induced by H₂O₂ Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana

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