A Rapid and Nondestructive Method for Soybean DNA Extraction and Its Application
Received date: 2015-03-31
Accepted date: 2015-09-25
Online published: 2016-02-01
Genotyping is an important work in plant genetic analysis and molecular marker-assisted breeding. This study established a simple method for genotyping soybean from mature seeds. By this method, a skilled worker can perform the sample collection and DNA extraction of 120 samples from soybean seeds in 1 h and the treated seeds can germinate normally. The DNA obtained by this method can meet the requirement for PCR amplification, and the success rate of PCR reaction is over 98%. This method can be widely used for identifying soybean hybrid seeds, seed purity and genotyping.
Key words： soybean seed; DNA extraction; nondestructive genotyping
Wen Cheng, Zhengjun Xia, Xianzhong Feng, Suxin Yang . A Rapid and Nondestructive Method for Soybean DNA Extraction and Its Application[J]. Chinese Bulletin of Botany, 2016 , 51(1) : 68 -73 . DOI: 10.11983/CBB15057
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